两种剂型的鱼肝油酸钠对ECV-304细胞系病理作用的对比研究

目的对比研究鱼肝油酸钠的普通剂型和脂质体剂型对ECV- 304细胞系的病理作用。方法以ECV- 304细胞系为实验对象，分别加入鱼肝油酸钠的普通剂型和脂质体剂型，通过对比形态学（ 倒置显微镜、Giemsa染色、透射电镜观察）、细胞活性（ MTT法）及流式细胞仪检测来探讨不同剂型对ECV- 304细胞系作用及其机制。结果普通剂型组出现细胞水肿、线粒体和内质网肿胀、染色质絮状化等坏死性变化；脂质体剂型组中染色质出现了新月状边集，碎裂并向胞膜移动，形成凋亡小体。细胞活性测定显示普通剂型组活细胞数目下降速度快；脂质体剂型组活细胞数目下降速度较慢。脂质体剂型组PI染色后行流式细胞仪检测，结果出现典型亚二倍峰。结论鱼肝油酸钠普通剂型表现为剧烈的毒性作用，呈现坏死性变化；而鱼肝油酸钠脂质体剂型对ECV- 304细胞系表现为渐进性的缓释作用，诱导细胞发生凋亡性变化。

Compare the Effect of Lipo- and Normal Prepared Sodium Morrhuate on ECV-304 Cell Line

Objective To compare the effect of Sodium Morrhuate on ECV-304 between its lipo-and normal preparation.Methods The ECV-304 cell line was supplemented with Sodium Morrhuate and lipo-Sodium Morrhuate in order,and the result on morphology(microscope,Giemsa Staining and electron microscope),cell activity(MTT),and flow cytometer between the two preparation were compared.Results In normal preparation group,cell's edema occurred.Chromatin was like catkins.Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared.In lipo-Sodium Morrhuate group,the membrane was creased and processus appeared.Chromatin aggregates to the membrane of nucleus was like crescent,and then broken.The apoptotic body was formed.MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively,which cues the vital cells decreased sharply.The curve of lipo-Sodium Morrhuate group was gentle and the change time was long relatively,which cues the vital cells decreased slowly.The flow cytometer showed that typical apoptosis peak appeared.Conclusion The normal preparation group shows an acute toxic effect on ECV-304 cell line,which result in a necrosis course,while lipo-Sodium Morrhuate shows a gradual releasing process,which may indicate a apoptosis course.