| Study on mitochondrial DNA diversity among 7 inbred strains of mice |
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| 作者姓名： | 戴纪刚 肖颖彬 张国强 闵家新 魏泓 |
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| 作者单位： | [1]Department of Thoracic Surgery , Xinqiao Hospital, Third Military Medical University , Chongqing 400037,China [2]Center of Laboratory Animal ,Third Military Medical University ,Chongqing 400038 ,China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);中国科学院资助项目 |
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| 摘 要： | Objective：To study the genetic variation of mitochondrial DNA （mtDNA） among common laboratory strains of inbred mice. Methods： The genetic polymorphism of mtDNA among 4 classical laboratory strains of inbred mice and 3 inbred strains of mice established in China was analyzed by polymerase chain reaction coupled with restriction fragment length polymorphism（PCR-RFLP） and PCR coupled with single-stranded conformation polymorphism（PCR-SSCP）. Results： With regard to the D-loop （Displacement loop, D-loop）, tRNA^ Met＋Glu＋Ile, and ND3 （NADH dehydrogenase subunit 3, ND3） gene fragments of mtDNA from these mice,no variation was revealed by PCR-RFLP at 46 restriction enzyme sites. Further analyzed by PCR-SSCP,the D-loop 5＇fragment and 3＇end fragment of mtDNA from these mice also showed no genetic variation. Conclusion： Owing to maternal mode of inheritance of mtDNA,the results indicate that these common inbred strains of mice share the same maternal lineage.
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| 关 键 词： | 线粒体 染色体 基因多样性 变应 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2006-02-25 |
Study on mitochondrial DNA diversity among 7 inbred strains of mice |
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| DAI Ji-gang,XIAO Ying-bin,ZHANG Guo-qiang,MIN Jia-xin,WEI Hong.Study on mitochondrial DNA diversity among 7 inbred strains of mice[J].Journal of Medical Colleges of PLA(China),2006,21(3):164-168. |
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| Authors: | DAI Ji-gang XIAO Ying-bin ZHANG Guo-qiang MIN Jia-xin WEI Hong |
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| Abstract: | Objective: To study the genetic variation of mitochondrial DNA(mtDNA) among common laboratory strains of inbred mice. Methods: The genetic polymorphism of mtDNA among 4 classical laboratory strains of inbred mice and 3 inbred strains of mice established in China was analyzed by polymerase chain reaction coupled with restriction fragment length polymorphism(PCR-RFLP) and PCR coupled with single-stranded conformation polymorphism(PCR-SSCP). Results: With regard to the D-loop(Displacement loop,D-loop),tRNAMet+Glu+(?)e,and ND3(NADH dehydrogenase subunit 3,ND3) gene fragments of mtDNA from these mice,no variation was revealed by PCR-RFLP at 46 restriction enzyme sites. Further analyzed by PCR-SSCP,the D-loop 5'fragment and 3'end fragment of mtDNA from these mice also showed no genetic variation. Conclusion: Owing to maternal mode of inheritance of mtDNA,the results indicate that these common inbred strains of mice share the same maternal lineage. |
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| Keywords: | inbred strains mtDNA polymerase chain reaction coupled with restriction fragment length polymorphism PCR coupled with single-stranded conformation polymorphism polymorphism |
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