| 牦牛皮胶氨基酸类成分的高效液相色谱指纹图谱 |
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| 引用本文： | 朱洪梅,魏立新,杜玉枝,肖远灿,杨红霞,李岑.牦牛皮胶氨基酸类成分的高效液相色谱指纹图谱[J].中成药,2012,34(2):195-198. |
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| 作者姓名： | 朱洪梅 魏立新 杜玉枝 肖远灿 杨红霞 李岑 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所藏药现代化研究中心,青海西宁810008;青海省藏药药理学与安全性评价研究重点实验室,青海西宁810008;中国科学院研究生院,北京100039
2. 中国科学院西北高原生物研究所藏药现代化研究中心,青海西宁810008;青海省藏药药理学与安全性评价研究重点实验室,青海西宁810008 |
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| 基金项目： | 青海省科学技术厅项目(2009GJG20044) |
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| 摘 要： | 目的 采用高效液相色谱法研究并建立牦牛皮胶氨基酸类成分的指纹图谱,为牦牛皮胶的质量控制提供有效的方法.方法 采用柱前衍生-RP高效液相色谱法,梯度洗脱进行分离,固定相为Agilent Analytical XDB-C18,(4.6 mm ×250mm,5μm)；流动相为pH6.4的醋酸钠缓冲液(A) - 60％乙腈(B),检测波长为360 nm,体积流量为1.0 mL/min,分析时间为48 min.结果 10批样品具有较高的相似度.天冬氨酸、谷氨酸、丝氨酸、甘氨酸、苏氨酸、脯氨酸、丙氨酸、缬氨酸、甲硫氨酸、异亮氨酸、亮氨酸、苯丙氨酸和赖氨酸的平均质量分数分别为5.58％、10.49％、3.35％、23.25％、1.74％、14.19％、8.98％、2.48％、0.29％、1.6％、3.07％、2.12％和3.57％.结论 该方法稳定可靠,可为其氨基酸质量的全面控制提供参考.
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| 关 键 词： | 牦牛皮胶 氨基酸 高效液相 指纹图谱 |
Development of HPLC fingerprints of amino acids constituents in yak collagen |
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| ZHU Hong-mei
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WEI Li-xin
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DU Yu-zhi
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XIAO Yuan-can
,
YANG Hong-xia
,
LI Cen.Development of HPLC fingerprints of amino acids constituents in yak collagen[J].Chinese Traditional Patent Medicine,2012,34(2):195-198. |
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| Authors: | ZHU Hong-mei WEI Li-xin DU Yu-zhi XIAO Yuan-can YANG Hong-xia LI Cen |
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| Institution: | 1,2,3(1.Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810008,China;2.Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation,Xining 810008,China;3.Graduate University of Chinese Academy of Sciences,Beijing 100039,China) |
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| Abstract: | AIM To develop an HPLC method for controlling the quality of yak collagen according to the amino acids constituents.METHODS The amino acids constituents were analysed by Pre-column derivatization reversed phase high performance liquid chromatography.Chromatographic conditions were as follows: Agilent Analytical XDB-C18 column(4.6 mm×250 mm,5 μm),mobile phase was natrium aceticum buffer solution(A)(pH = 6.5)-60%acetonitrie(B),the detective wavelength was set at 360 nm,the flow rate was 1 mL /min and the analytical time was 48 minutes.RESULTS The result showed that the amino acids constituents had a high similarity.The contents of Asp,Glu,Ser,Gly,Thr,Arg,Ala,Val,Met,Ile,Leu,Phe and Lys were 5.58%,10.49%,3.35%,23.25%,1.74%,14.19%,8.98%,2.48%,0.29%,1.6%,3.07%,2.12% and 3.57%,respectively.CONCLUSION The method has good reproducibility and stability,and can be served as the means to control the quality of yak collagen. |
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| Keywords: | yak collagen amino acids HPLC fingerprints |
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