| 胃癌和消化性溃疡患者幽门螺杆菌临床菌株硫氧还蛋白1表达量分析 |
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| 引用本文： | 石岩岩,丁士刚,鲁凤民,张静,刘琳娜,王晔. 胃癌和消化性溃疡患者幽门螺杆菌临床菌株硫氧还蛋白1表达量分析[J]. 胃肠病学, 2011, 16(10): 601-604. DOI: 10.3969/j.issn.1008-7125.2011.10.007 |
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| 作者姓名： | 石岩岩 丁士刚 鲁凤民 张静 刘琳娜 王晔 |
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| 作者单位： | 1. 北京大学第三医院消化科 100191
2. 北京大学医学部病原生物学系 100191 |
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| 基金项目： | 国家自然科学基金项目(No.30770980)资助 |
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| 摘 要： | 背景：幽门螺杆菌（H．pylori）与胃部疾病密切相关，多种H．priori毒力因子参与其致病，前期研究发现分离自不同胃部疾病的Hpylri临床菌株硫氧还蛋白（亿）表达水平存在明显差异。目的：比较分离自不同胃部疾病的H．pvlori临床菌株的TrxlmRNA表达情况。方法：胃镜获取胃窦黏膜组织，分离培养获得15例慢性胃炎、13例消化性溃疡、13例胃癌患者的H．pylori临床菌株。实时荧光定量RT—PCR检测各组H．priori临床菌株的Trx1mRNA表达量．并筛选Trx1高、低表达量界值。结果：胃癌组和消化性溃疡组H．priori临床菌株的Trx1mRNA表达量明显高于慢性胃炎组（P〈0．05），胃癌组与消化性溃疡组间比较差异无统计学意义（P〉0．05）。△Ct的90％CI为8．86～28．05．即ACt〈8．86为Trx1高表达．ACt〉28．05为Trx1低表达。结论：分离自胃癌和消化性溃疡患者的H．pylori临床菌株高表达Trx1mRNA，提示其可能与H.pylori以的致病性有关。筛选的Trx1高低表达量界值可用于后续研究。
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| 关 键 词： | 螺杆菌，幽门 硫氧还蛋白 胃肿瘤 |
Expression of Thioredoxin 1 in Strains of Helicobacter pylori Isolated from Patients with Gastric Cancer and Peptic Ulcer |
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| SHI Yanyan,DING Shigang,LU Fengmin,ZHANG Jing,LIU Linna,WANG Ye. Expression of Thioredoxin 1 in Strains of Helicobacter pylori Isolated from Patients with Gastric Cancer and Peptic Ulcer[J]. Chinese Journal of Gastroenterology, 2011, 16(10): 601-604. DOI: 10.3969/j.issn.1008-7125.2011.10.007 |
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| Authors: | SHI Yanyan DING Shigang LU Fengmin ZHANG Jing LIU Linna WANG Ye |
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| Affiliation: | SHI Yanyan,DING Shigang,LU Fengmin,ZHANG Jing,LIU Linna,WANG Ye.Department of Gastroenterology,Peking University Third Hospital,Beijing(100191) |
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| Abstract: | Background:Helicobacter pylori(H.pylori) has a close relationship with gastric diseases,and multiple virulence factors are involved in its pathogenicity.Preliminary studies have shown that the expression of thioredoxin(Trx) was different among strains of H.pylori isolated from various gastric diseases.Aims:To compare the expression of Trx1 mRNA in strains of H.pylori isolated from various gastric diseases.Methods:Gastroscopy was performed to obtain antral mucosa specimens and strains of H.pylori were isolat... |
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| Keywords: | Helicobacter pylori Thioredoxin Stomach Neoplasms |
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