| XIAP通过调节CDK4／CDK6／cyclinD1复合物表达促进肝癌细胞增殖 |
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| 引用本文： | 车宇芳,叶飞,青海涛,姜泊,张泳. XIAP通过调节CDK4／CDK6／cyclinD1复合物表达促进肝癌细胞增殖[J]. 胃肠病学和肝病学杂志, 2011, 20(10): 939-944 |
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| 作者姓名： | 车宇芳 叶飞 青海涛 姜泊 张泳 |
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| 作者单位： | 1. 南方医科大学附属南方医院消化内科,广东广州,510515
2. 美国西奈山医学院 |
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| 摘 要： | 目的研究肝癌细胞中XIAP与cDK4／cDK6／cyclinD1复合物之间的调控关系及在肝癌细胞增殖中的作用。方法前期研究用PathwayArray进行肝癌组织异常表达蛋白筛选，后期XIAP特异性抑制剂embelin处理Huh7细胞进行增殖、周期分析。Westernblot验证XIAP与cDK4／cDK6／cyclinD1复合物之间的调控关系。结果PathwayArray提示XIAP与CDK4、CDK6、cyclinD1之间存在联合表达。embelin引起Huh7细胞G1期阻滞、S期分布减少，抑制细胞增殖。XIAP可以调节Huh7细胞G1期蛋白CDK4、CDK6、cyclinD1表达。结论XIAP通过调节肝癌细胞G1期蛋白CDK4／CDK6／eyelinD1复合物的表达，促使细胞进入G1周期，从而促进其增殖。
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| 关 键 词： | XIAP CDK4 CDK6 cyclin D1 肝癌细胞 增殖 |
XIAP promote cell proliferation of hepatocellular carcinoma through regulating expression of CDK4/CDK6/cyclin D1 complex |
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| CHE Yufang,YE Fei,QING Haitao,JIANG Bo,ZHANG Yong. XIAP promote cell proliferation of hepatocellular carcinoma through regulating expression of CDK4/CDK6/cyclin D1 complex[J]. Chinese Journal of Gastroenterology and Hepatology, 2011, 20(10): 939-944 |
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| Authors: | CHE Yufang YE Fei QING Haitao JIANG Bo ZHANG Yong |
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| Affiliation: | CHE Yufang1,YE Fei2,QING Haitao1,JIANG Bo1,ZHANG Yong2 1.Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,2.Mount Sinai School of Medicine,New York |
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| Abstract: | Objective To investigate the relationship between XIAP and CDK4/CDK6/cyclin D1 complex in cell growth of hepatocellular carcinoma(HCC).Methods In preliminary study,Pathway Array was used to analyze protein in HCC tissues.After treatment of embelin in Huh7 cells,cell proliferation and cell cycle analysis were proceeded.Protein expression of XIAP,CDK4,CDK6 and cyclin D1 were detected by Western blot.Results Pathway Array showed co-expression of XIAP with CDK4,CDK6,cyclinD1 in HCC tissues.Embelin could lead ar... |
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| Keywords: | XIAP CDK4 CDK6 Cyclin D1 HCC Proliferation |
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