| Nucleostemin在结直肠癌和正常结直肠组织中的表达及其意义 |
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| 引用本文： | 范里,朱良如.Nucleostemin在结直肠癌和正常结直肠组织中的表达及其意义[J].胃肠病学,2011,16(10):585-588. |
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| 作者姓名： | 范里 朱良如 |
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| 作者单位： | 1. 济宁医学院附属医院消化内科 272029
2. 华中科技大学同济医学院附属协和医院消化内科 430022 |
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| 摘 要： | 背景：Nucleosterain（NS）是一个与细胞增殖相关的核蛋白，部分研究显示其仅高表达于干细胞和肿瘤细胞，成体组织分化细胞中未见NS表达。目的：探讨NS在结直肠癌和正常结直肠组织中的表达及其意义。方法：收集38例结直肠癌患者的癌组织及其相应癌旁正常组织标本．以实时RT—PCR和蛋白质印迹法检测NSmRNA和蛋白表达。结果：实时PCR扩增曲线显示结直肠癌组织和正常结直肠组织均有NS基因指数式扩增，癌组织NSmRNA表达量显著高于正常组织（1．8939±0．9529对1．P=0．011）。蛋白质印迹法证实NS蛋白在正常组织中表达较微弱或几乎无表达，癌组织中其表达显著增高（1．5397±0．3625对0．6336±0．3443，P=0．001）。结论：结直肠癌组织和正常结直肠组织中均存在NS基因．推测其微量表达可调节细胞正常增殖．而异常高表达则可通过某些机制促进细胞恶性增殖。
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| 关 键 词： | Nucleostemin 细胞增殖 结直肠肿瘤 实时PCR 印迹法，蛋白质 |
Expression and Significance of Nucleostemin in Cancerous and Normal Colorectal Tissues |
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| FAN Li,ZHU Liangru.Expression and Significance of Nucleostemin in Cancerous and Normal Colorectal Tissues[J].Chinese Journal of Gastroenterology,2011,16(10):585-588. |
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| Authors: | FAN Li ZHU Liangru |
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| Institution: | FAN Li,ZHU Liangru. Department of Gastroenterology,Union Hospital,Tongji Medical College of Huazhong University of Science & Technology,Wuhan(430022) |
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| Abstract: | Background:Some studies revealed that nucleostemin(NS),a cell proliferation-related protein,was highly and exclusively expressed in the nucleus of stem cells and tumor cells,and was not expressed in differentiated cells of adult tissue.Aims:To investigate the expression and significance of NS in cancerous and normal colorectal tissues. Methods:Cancerous and adjacent normal tissues of 38 colorectal carcinoma patients were collected.Real-time RT-PCR and Western blotting were used to detect the expressions of ... |
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| Keywords: | Nucleostemin Cell Proliferation Colorectal Neoplasms Real-Time PCR Blotting Western |
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