| 马来酸曲美布汀对结肠平滑肌细胞钙激活钾通道的影响 |
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| 引用本文： | 张虹,罗和生,谭(偉). 马来酸曲美布汀对结肠平滑肌细胞钙激活钾通道的影响[J]. 胃肠病学和肝病学杂志, 2011, 20(10): 920-923 |
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| 作者姓名： | 张虹 罗和生 谭(偉) |
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| 作者单位： | 1. 武汉市三医院
2. 武汉大学人民医院消化内科,湖北武汉,430060 |
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| 摘 要： | 目的研究马来酸曲美布汀对豚鼠结肠平滑肌细胞膜钙激活钾通道的影响。方法酶解法急性分离单个豚鼠结肠平滑肌细胞，利用膜片钳技术在全细胞模式下记录钙激活钾电流[IK（ca）]，用含有IK（ca）特异激动剂NS1619的生理盐水灌流细胞，使结肠平滑肌细胞处于超极化状态，检测给马来酸曲美布汀浓度分别为1．19μmol／L、5．95μmol／L、11．91μmol／L后的结肠平滑肌细胞膜IK（ca）。结果浓度为1．19μmol／L、5．95μmol／L、11．91μmol／L的马来酸曲美布汀可抑制豚鼠单个结肠平滑肌细胞膜IK（ca）（P均〈0．01），当阶跃刺激为＋80mv时，其分别为超极化状态组的（65．87±4．80）％、（48．02±2．39）％、（18．88±2．29）％（P均〈0．01）。结论马来酸曲美布汀能抑制豚鼠结肠平滑肌细胞钙激活钾通道的开放，使细胞兴奋性升高，且呈浓度依赖性，这可能是马来酸曲美布汀治疗肠易激综合征便秘症状的机制之一。
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| 关 键 词： | 马来酸曲美布汀 钙激活钾通道 结肠平滑肌细胞 |
Effect of trimebutine on the calcium activated potassium channel of colonic smooth muscle cells |
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| ZHANG Hong,LUO Hesheng,TAN Wei. Effect of trimebutine on the calcium activated potassium channel of colonic smooth muscle cells[J]. Chinese Journal of Gastroenterology and Hepatology, 2011, 20(10): 920-923 |
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| Authors: | ZHANG Hong LUO Hesheng TAN Wei |
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| Affiliation: | ZHANG Hong,LUO Hesheng,TAN Wei Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China |
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| Abstract: | Objective To study the effect of trimebutine on the calcium activated potassium channel of colonic smooth muscle cells(SMCs).Methods Smooth muscle cells were isolated freshly from the colon strips of guinea pig by enzymolysis,the calcium activated potassium currents were recorded by patch clamp technique at whole cell model.The SMCs were perfused with physiologicalsaline(PSS) which contained NS1619-the calcium activated potassium channel's selective activator,causing the SMCs to be at the state of hyperpola... |
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| Keywords: | Trimebutine Calcium activated potassium channel Colonic smooth muscle cells |
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