四磨汤对大鼠胃窦平滑肌影响及其机制的研究

临床证实四磨汤可改善胃肠动力障碍疾病的症状。但机制不明。目的：研究四磨汤对大鼠离体胃窦平滑肌收缩活动的影响及其机制。方法：处死Sprague-Dawlev大鼠后收集胃窦纵行和环行平滑肌条，检测不同剂量四磨汤（1μl、5μl、25μl、50μl、100μl、150μl和200μl）对胃窦平滑肌收缩的影响，同时观察M受体阻断剂阿托品（100mol／L）、M受体激动剂乙酰胆碱（10-6mol／L）对四磨汤诱导的胃窦平滑肌收缩的影响。结果：低一中剂量（1～100μl）四磨汤剂量依赖性地诱导大鼠胃窦纵行肌和环行肌收缩增强，但两者之间的作用无明显差异。阿托品可完全阻断四磨汤对胃窦环行肌、纵行肌的促收缩作用，且对环行肌的阻断效应更明显。以乙酰胆碱预处理后，四磨汤对胃窦纵行肌和环行肌的促收缩作用进一步增强．且对环行肌的作用更明显。结论：四磨汤对大鼠胃窦纵行肌和环行肌具有明显的促收缩作用．该作用主要通过M受体介导。

Study on Effect and Mechanism of Simo Decoction on Gastric Antral Smooth Muscle in Rats

Background:Simo Decoction has been proved having the effect of improving symptoms of gastrointestinal dysmotility in clinical studies,but the mechanism is unclear.Aims:To investigate the effect and mechanism of Simo Decoction on the contractive activity of isolated gastric antral smooth muscle in rats.Methods:Antral longitudinal and circular muscle strips were prepared after sacrificing Sprague-Dawley rats.The effect of different concentrations of Simo Decoction（1μl,5μl,25μl,50μl,100μl,150μl,200μl） on gastric antral smooth muscle contraction was determined, and the effects of M receptor inhibitor atropine(10^-6 mol/L) and M receptor agonist acetylcholine(10^-6 mol/L) on gastric antral smooth muscle contraction induced by Simo Decoction were assessed.Results:Low to moderate doses（1-100μl） of Simo Decoction dose-dependently increased the contraction of gastric antral longitudinal and circular muscle,and no significant difference was found between longitudinal and circular muscle.After pre-treatment with atropine,the increased contraction of longitudinal and circular muscle induced by Simo Decoction could be completely blocked,especially the circular muscle;however,if pre-treatment with acetylcholine,Simo Decoction further increased the contraction,especially the circular muscle.Conclusions:Simo Decoction can dose-dependently promote the contraction of gastric antral longitudinal and circular muscle,and this is mainly mediated by activation of M receptor.