双标稳定性同位素测定微量元素吸收率

迄今为止 ,测定微量元素吸收率较准确的方法依然是同位素法。由于放射线同位素的危害性 ,使得放射线同位素在某些人群中的应用受到限制。随着稳定性同位素技术的发展 ,应用稳定性同位素来研究微量元素的吸收和利用成为近年来在微量元素营养研究中的热点。特别是双标稳定性同位素技术更以其准确性高而受到微量元素学家的关注。然而目前普遍使用的计算方法多数以估计的机体血液体积及血红蛋白掺入率为基础 ,从而人为地引入了误差。为了更为有效地利用已有资料、准确地计算微量元素的生物利用 ,在前人工作的基础上 ,作者在本文对使用双标稳定性同位素技术测定微量元素的吸收率的方法进行了较为详细地讨论 ,对该方法的理论假设、实验方法、所需的基础数据、计算思路及其具体的计算过程都作了具体的说明。本文所介绍的方法可方便地用于具体的实验中

Mineral absorption from dual stable isotopic technique

Isotope technique is the most accurate method to assess the bioavailability of trace elements. Due to the hazard of radioisotopes, it can't be used to such populations as infants and pregnant women. With the development of stable isotope technique, more and more researches were conducted with stable isotopes. The newly developed dual stable isotope technique was preferred because of its accuracy. While the papers published using such technique till now usually contained the estimation of the blood volume and hemoglobin incorporation. Because of such estimation, errors would be introduced into the results. To obtain the accurate result from the data available, this paper described a rigorous method to calculate the trace element bioavailability. The dual stable isotopic technique was discussed in detail in this paper. From the assumption of the method to their performance, the author described the procedure of calculation of the mineral absorption from the MS data and why. This method was described by using iron as an example, and can be used in similar studies.