祖师麻注射液中紫丁香苷和祖师麻甲素的含量测定

目的建立测定祖师麻注射液中紫丁香苷和祖师麻甲素含量的高效液相色谱（HPLC）法。方法色谱柱为AgilentZorbax SB—C18柱（250mm×4．6mm，5um），流动相为乙腈-0．05％磷酸溶液（梯度洗脱），梯度变化检测波长为223nm和327nm，流速为1．00mL／min，柱温为30℃。结果祖师麻甲素、紫丁香苷进样量分别在0．1035～6．2100ug和0．01979～0．59376txg范围内与其峰面积线性关系良好。平均回收率分别为93．14％和92．07％，RSD分别为2．16％和1．70％（n=6）。结论HPLC法简便准确、重现性好，可用于产品的质量控制。

Determination of Daphnetin and Syringin in Zushima Injection by HPLC

Objective To study and establish a HPLC method for the content determination of daphnetin and syringin in Zushima Injection. Methods The Agilent Zorbax SB-C18 column（250 mm×4.6 mm, 5um） was used at the temperature of 30 ℃.The mobile phase consisted of acetonitile-0. 05% phosphoric acid with gradient elution and the detection wavelength at 223 nm and 327 nm, the flow rates was 1.00 mL/min. Results The calibration curve of daphnetin and syringin were in linear over the range of 0. 103 5-6. 210 0ug and 0. 019 79 -0. 593 76 ug, respectively. The average recovery rates were 93.14% and 92.07%, RSD was 2.16% and 1.70%, respectively. Conclusion The method is simple and accurate with good reproducibility, which can be used for the quality control of Zushima Injection.