喉癌患者外周血NK细胞杀伤活性及T淋巴细胞亚群的改变

目的 :探讨喉癌患者外周血NK细胞 (NKC)杀伤活性及T淋巴细胞亚群的改变 ,以评估患者的免疫机能 ,预测预后。方法 :采用乳酸脱氢酶 (LDH)释放法 ,测定NKC杀伤活性 ;应用流式细胞仪检测T淋巴细胞亚群。结果 :喉癌组外周血NKC杀伤活性明显低于正常对照组及声带息肉组 (P <0 .0 5 ) ,病变程度越重 ,NKC杀伤活性越低。喉癌患者术后 3～ 6dNKC杀伤活性明显低于术前 (P <0 .0 5 ) ,7d后呈上升趋势。在NKC杀伤活性降低的同时 ,伴随CD3 、CD4及CD4/CD8比值下降 ,与正常对照组相比较 ,其差异有显著性意义 (P <0 .0 5 )。结论 :检测喉癌患者外周血NKC杀伤活性及T淋巴细胞亚群可客观反映机体免疫状态 ,具有重要临床意义

Changes of NK cell's activity and T lymphocyte subpopulation in periferal blood of patients with laryngocarcinoma

OBJECTIVE: By detecting the changes of NK cell's activity and T lymphocyte subpopulation in the peripheral blood of the patients with laryngocarcinoma, the immune function level of the patients could be judged and the prognosis of the patients could be predicted. METHOD: The NK cell's activity in 47 patients with laryngocarcinoma was tested, and T lymphocyte subpopulation in 35 patients were detected with flow cytometer, and compared with the normal control group of 45 volunteers and the group of 15 patients with polyp of vocal cord. RESULT: The NK cell's activity in the patients with laryngocarcinoma was lower than both of the normal control group and the polyp of vocal cord patients group, and the more severe was the degree of disease, the lower of the NK cell's activity was. The NK cell's activity in the pharyngocacinoma patients group in the 3-6 days after operation was lower than that in preoperation significantly; in the same time when the NK cell's activity was droping, CD3 and the ratio of CD4/CD8 became lower. CONCLUSION: By detecting the changes of both NK cell's activity and the T lymphocyte subpopulation in patients with laryngocarcinoma, the immune function level of the patients can be judged objectively, which is of the important clinic significance.