|
|||||
|
|
| 硒抑制镍诱导的人肺成纤维细胞的DNA损伤 | |
| 引用本文： | 张敬 张军 石红军 李卓权 郭超 汪丽丽. 硒抑制镍诱导的人肺成纤维细胞的DNA损伤[J]. 同济大学学报(医学版), 2004, 25(3): 189-191 |
| 作者姓名： | 张敬 张军 石红军 李卓权 郭超 汪丽丽 |
| 作者单位： | 1. 同济大学生命科学与技术学院,上海,200331 2. 同济大学医学院生物教研室,上海,200070 3. 同济大学医学院2000级医疗系,上海,200331 |
| 基金项目： | 铁道部科研基金资助项目(J99022) |
| 摘 要： | 目的 探讨三氧化二镍(Ni2O3)对人肺成纤维细胞(human lung fibroblasts,HLF)的DNA损伤作用以及Na2SeO3的保护作用。方法 应用单细胞凝胶电泳(single cell gel electrophoresis,SCGE)检测DNA损伤。结果 Ni2O3 处理HLF细胞4 h,SCGE检测出DNA损伤程度高于对照组,差异有显著性(P<0.01)。而Ni2O3 Na2SeO3 处理组DNA损伤程度低于Ni2O3 组,差异有显著性(P<0.01)。结论 硒可抑制镍诱导的人肺成纤维细胞的DNA损伤。 |
| 关 键 词： | 亚硒酸钠 三氧化二镍 DNA损伤 单细胞凝胶电泳 |
| 文章编号： | 1008-0392(2004)03-0189-03 |
| 修稿时间： | 2004-03-20 |
The Inhibited Effects of Selenium on DNA Damage Induced by Nickel in Human Lung Fibroblasts |
|
| ZHANG Jing,ZHANG Jun,SHI Hong-jun,LI Zhuo-quan,GUO Chao,WANG Lui. The Inhibited Effects of Selenium on DNA Damage Induced by Nickel in Human Lung Fibroblasts[J]. Journal of Tongji University(Medical Science), 2004, 25(3): 189-191 | |
| Authors: | ZHANG Jing ZHANG Jun SHI Hong-jun LI Zhuo-quan GUO Chao WANG Lui |
| Affiliation: | ZHANG Jing1,ZHANG Jun1,SHI Hong-jun2,LI Zhuo-quan,GUO Chao,WANG Lui |
| Abstract: | Objective To study the mechanisms of inhibited effects of Na 2SeO 3 on DNA damage induced by Ni 2O 3 in human lung fibroblasts (HLF) cells.Methods Human lung fibroblasts were cultured in vitro and treated with Ni 2O 3 and Ni 2O 3 Na 2SeO 3,respectively.The single cell gel electrophoresis to detect DNA breakage induced by Ni 2O 3 was carried out.Results The level of DNA breakage induced by Ni 2O 3 was higher than the control group(P<0.01). But the level of DNA breakage induced by Ni 2O 3 Na 2SeO 3 was lower than Ni 2O 3 -treated group (P<0.01).Conclusion Na 2SeO 3 can scavenge the free radicals induced by Ni 2O 3 and prevent cells from DNA damage. |
| Keywords: | Ni 2O 3 Na 2SeO 3 DNA damage single cell gel electrophoresi |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《同济大学学报(医学版)》浏览原始摘要信息 | |
| 点击此处可从《同济大学学报(医学版)》下载全文 | |