Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates |
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| Authors: | Løvseth Astrid Loncarevic Semir Berdal Knut G |
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| Affiliation: | National Veterinary Institute, Section of Feed and Food Microbiology, P.O. Box 8156 Dep., 0033 Oslo, Norway. astrid.lovseth@vetinst.no |
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| Abstract: | A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results. |
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