Interaction of protamine sulfate with thrombin |
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Authors: | R J Cobel-Geard H I Hassouna |
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Institution: | 1. Upstate Medical Center, Syracuse, New York;2. Roswell Park Memorial Institute, Buffalo;3. University of Rochester, Rochester, New York;4. Emory University, Atlanta;5. New England Medical Center, Boston;6. Queens Hospital Center, Queens, New York |
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Abstract: | Protamine sulfate (salmine), a basic protein with a molecular weight of 4,626 +/- 109, is a known antiheparin agent which in the absence of heparin demonstrates an anticoagulant activity. To date, much work has been done to elucidate the interaction of heparin with thrombin and its physiologic inhibitor, Antithrombin III (ATIII). Little is known, however, about the mechanism of anticoagulant action of protamine sulfate and its mode of thrombin inactivation. We provide information about the interaction of protamine sulfate with purified, labeled thrombin and ATIII through binding experiments in which protamine is shown to inhibit the inactivation of thrombin by ATIII. Furthermore, we show in clotting assays that protamine sulfate has an inhibitory effect on thrombin in the conversion of fibrinogen to fibrin, and that this inhibition is concentration dependent, partial, and reversible. |
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Keywords: | protamine sulfate inhibitor thrombin |
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