RP-HPLC法测定人血浆中莫西沙星的浓度

目的:建立以反相高效液相色谱法测定人血浆中莫西沙星浓度的方法。方法:色谱柱为Gemini C18,固定进样环为20μL,流动相为甲醇-水-三乙胺(112.5∶208.9∶0.06),流速为1mL·min-1,紫外检测波长为289nm,柱温为25℃,灵敏度为0.01AUFS。结果:莫西沙星检测浓度在0.3125~10μg·mL-1范围内线性关系良好(r=0.9998);其低、中、高3种浓度(10、2.5、0.625μg.mL-1)的回收率分别为99.46%、103.12%、96.14%;日内RSD分别为3.27%、3.35%、0.92%,日间RSD分别为7.19%、6.25%、6.68%。结论:本方法简单、快速、准确、灵敏度高,可用于莫西沙星的血药浓度测定及药动学研究。

Determination of Moxifloxacin in Human Serum by RP - HPLC

OBJECTIVE:To establish a RP-HPLC method for the determination of the concentration of moxifloxacin in human serum.METHODS:The separation of sample was performed on Gemini C18 column with a fixed sample injection volume of 20μL.The mobile phase was methanol-water-triethylamine(112.5∶208.9∶0.06) at a flow rate of 1mL·min-1.The UV detective wavelength was set at 289nm;the column temperature was 25℃ and the sensitivity was 0.01AUFS.RESULTS:In the range of 0.312 5～10μg·mL-1(r=0.999 8),moxifloxacin showed a good linear relation in HPLC.The recoveries of moxifloxacin at three concentrations(10,2.5,0.625μg·mL-1) were 99.46%,103.12%,and 96.14%,respectively,with intra-day RSD at 3.27%,3.35%,0.92%,respectively and inter-day RSD at 7.19%,6.25%,6.68%,respectively.CONCLUSION:This method is simple,rapid,accurate and sensitive,and it can be used for the concentration determination and the pharmacokinetic study of moxifloxacin.