LTB4诱导THP-1巨噬细胞MMP-9表达及在急性冠脉综合征中的意义 |
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引用本文: | 葛卫力,唐礼江,江建军,朱敏,方崇峰,王斌,米亚非,王教辰. LTB4诱导THP-1巨噬细胞MMP-9表达及在急性冠脉综合征中的意义[J]. 心脑血管病防治, 2010, 10(4): 259-262,258. DOI: 10.3969/j.issn.1009-816X.2010.04.03 |
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作者姓名: | 葛卫力 唐礼江 江建军 朱敏 方崇峰 王斌 米亚非 王教辰 |
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作者单位: | 温州医学院附属台州医院,浙江,临海,317000 |
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基金项目: | 浙江省科技厅基金资助项目 |
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摘 要: | 急性冠脉综合征(Acute Coronary Syndrom,ACS)包括急性心肌梗死(AMI)、不稳定心绞痛(UA)和心源性猝死,是严重冠状动脉粥样硬化性心脏病的表现形式,其病理生理机制尚未明确,炎症机制是目前研究的热点。白三烯B4(Leukotriene B4,LTB4)激活巨噬细胞产生炎症介质,与动脉粥样斑块不稳定关系密切;MMP-9(Matrix Metalloproteinases,MMP-9)降解细胞外基质而成为致斑块易损的重要炎症因子。
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关 键 词: | THP-1巨噬细胞 急性冠脉综合征 LTB4 MMP-9 冠状动脉粥样硬化性心脏病 动脉粥样斑块 急性心肌梗死 不稳定心绞痛 |
Induced MMP-9 Expression by Leukotriene B4(LTB4) in THP-1 Macrophages and Its Significance in Patients with Acute Coronary Syndrome(ACS) |
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Affiliation: | GE Wei-li,TANG Li-jiang,JIANG Jian-jun,et al.(Department of Cardiology,Tauzhou Hospital,Affiliated Hospital of Wenzhou Medical College,Zhejiang 317000 China ) |
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Abstract: | Objective To observe the expression of Matrix Metalloproteinase-9 (MMP-9) induced by LTB4 in THP-1 macrophages and to explore its significance in patients with ACS.Methods Differentiated THP-1 macrophages was stimulated with LTB4 in different concentration(10-10~10-7mol/L) and for different time(0,6,12,24 and 48 hours).RT-PCR was used to measure the expression of MMP-9 mRNA and Gelatin Zymography was used to evaluate the activity of MMP-9.Serum LTB4,MMP-9 and CRP was measured by Enzyme-linked immunosorbent assay(ELISA) in 115 patients with ACS and 105 healthy people(control group).Results LTB4(10^-10~10^-7mol/L) upregulated MMP-9 mRNA expression in dose-and time-dependent manner and also enhanced MMP-9 activity.Serum LTB4 was significant higher in patients with acute myocardial infarction(AMI)and unstable angina pectoris(UAP) than that in control group(112.77±22.59pg/mL,86.94±14.81pg/mL and 40.5±8.85pg/mL,respectively.P〈0.01).Besides,LTB4 was positive correlated with MMP-9(r=0.875,P〈0.001)and CRP(r=0.641,P〈0.01).Conclusions LTB4 significantly upregulates MMP-9 expression and enhances its activity.Furthermore,levels of LTB4 and MMP9 are elevated in ACS.LTB4 and MMP-9 may play an important role in atherosclerosis by combining to form an inflammatory network and thus may contribute to the attack of ACS and serve as a potential treating target. |
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Keywords: | THP-1 LTB4 MMP-9 ACS |
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