大鼠骨癌痛模型的建立及组织学研究

目的建立一个有用的癌痛动物模型.方法将MRMT-1大鼠乳腺癌细胞接种到SD大鼠胫骨上段骨髓腔内,造成骨癌痛动物模型.用von Frey细丝刺激足底和后肢负重测量仪分别测量机械性痛觉超敏和痛觉过敏,放射学方法评估骨破坏,同时,组织切片镜下观察肿瘤和骨结构的破坏情况.结果造模动物在14天左右开始出现机械性痛觉超敏和痛觉过敏,胫骨X线摄片显示明显的骨破坏,组织学研究显示骨髓腔内肿瘤生长,向外侵蚀破坏骨皮质,同时伴有新生的编织骨形成.结论从疼痛行为学、放射学、组织学多方面研究的结果,表明大鼠骨癌痛模型已复制成功.该癌痛模型的建立,将为我国癌痛新药的评价,尤其是研究治疗癌痛中药的疗效及作用机制提供一个有用的工具.

Study on the histopathology in a rat model of bone cancer pain

Objective To establish a useful animal model of cancer pain.Methods A rat model of bone cancer pain was developed by intra-tibial inoculations of MRMT-1 rat mammary gland carcinoma cells in Sprague-Dawley rats.Mechanical allodynia was measured by using von Frey test,mechanical hyperalgesia was assessed as the difference of weight bearing between hind paws,measured using a incapacitance tester.The development of the bone tumor and structural damage to the bone was monitored by radiological analysis and histology.Results Rats following intra-tibial injections of MRMT-1 cells displayed the gradual development of mechanical allodynia and hyperalgesia,beginning on day 14 after injection of the tumor cells.The radiograph of the tibia showed clear bone destruction.The sections of the bone showed the bone marrow cavity was full of tumor cells,the cortical bone has been destroyed and replaced by woven bone.Conclusion Results from the studies on behavioral tests,radiology and histology suggest that this model of bone cancer has been copied well,and it will provide us a useful animal model for evaluation of new drugs of cancer pain.