儿童近视与IGF1及IGFBP-3的相关研究

目的：通过检测正常儿童及近视儿童血清中血清胰岛素样生长因子1（insulin like growth factor 1, IGF1）和结合蛋白-3（insulin like growth factor binding protein-3,IGFBP-3）的浓度，研究它们对儿童眼球发育的调控及该调控作用对儿童近视及近视程度的相关性。方法：近视组：随机选择我院眼科门诊的5～14岁近视眼儿童60例120眼；对照组：同年龄段的正常儿童60例120眼。通过常规眼部检查及散瞳检影验光。按近视程度分为轻度近视，-3.00D以下；中度近视，-3.00～-6.00D；高度近视，-6.00D以上。所有研究对象均采用ELISA法测定血清IGF1，IGFBP-3的浓度。统计学方法采用SPSS 13.0软件包，近视组和对照组的特征差异采用t检验或χ2检验。近视组均数间两两比较采用LSD-t检验。两变量的相关分析采用Pearson分析。结果：两组资料特征差异（年龄、性别、身高、体质量、角膜屈光力、角膜中央厚度）统计学上差异无显著性（P>005），而近视组眼轴长度高于对照组，差异有显著性（P<0.05）。近视组血清IGF1，IGFBP-3浓度明显高于对照组。两组比较差异有显著性（P<0.01）；近视程度越高，血清中IGF1，IGFBP-3浓度也越高，两两比较差异均有显著性（P<0.05）；血清IGF1，IGFBP-3浓度与近视程度、眼轴长度、屈光度呈正相关（P<0.01），而与身高、体质量、角膜屈光力、角膜中央厚度无明显关系（P>0.05）。结论：实验研究进一步证实了血清IGF1，IGFBP-3浓度对儿童眼球发育起着调控作用，而该调控与儿童近视的发生、发展之间存在内在相关性； IGF1的生物活性受IGFBP-3的调节。

Research of correlation between children myopia and insulin-like growth factor1 and insulin-like growth factor binding protein-3

AIM:To study its controlling effect of ocular development and correlation of controlling effect to the degree of myopia by observing concentration of insulin like growth factor 1(IGF1) and insulin like growth factor binding protein-3(IGFBP-3) in children with myopia and emmetropia. METHODS: Sixty children 120 eyes with myopia and 60 children 120 eyes with emmetropia were collected at random. All subjects with myopia were examined and separated into three groups according to visual acuity: mild myopia worse than -3.00D, moderate myopia(-3.00 --6.00D), high myopia(better than -6.00D). IGF1 and IGFBP-3 of all subjects were measured with enzyme linked immunosorbent assay (ELISA). The data were analyzed by SPSS 13.0 statistics software. RESULTS:There was no statistical difference between two groups in age, gender, height, weight, corneal refractive power and corneal thickness. The ocular axial length of myopia group was longer than control group. IGF1 and IGFBP-3 concentration of myopia group were higher than control group. The more degree of myopia,the higher IGF1 and IGFBP-3 concentration . There were positive correlations between IGF1 or IGFBP-3 and the degree of myopia, ocular length, refraction. There were no correlations between IGF1 or IGFBP-3 and height, weight, corneal refractive power, corneal thickness. CONCLUSION: Experiments further confirm controlling effect of IGF1 and IGFBP-3 concentration on ocular development. There exist the correlations between the controlling effect and the generation and development of myopia. Biological activity of IGF1 is controlled by IGFBP-3.