miR-205对人胃癌细胞株SGC7901增殖的抑制作用

摘要：目的：观察miR-205对人胃癌细胞株SGC7901增殖的影响及可能的机制。 方法：miR-205 mimics转染SGC7901细胞后，观察其增殖能力；SYBR Green I实时荧光定量PCR检测miR205的表达水平；RT-PCR检测细胞ZEB1和ZEB2的基因表达水平。 结果：与空白对照组miR-205表达的相对水平（0.000 619±0.000）及阴性片段对照组（0.001 064±0.001）相比，miR-205 mimics处理组(0.027 33±0.014)miR205表达水平明显增高（q分别为5.77和5.67，P均<0.01），而SGC7901细胞的克隆形成能力明显下降，ZEB1和ZEB2 mRNA明显下调。 结论：miR-205可抑制胃癌细胞增殖，其机制可能与下调ZEB1和ZEB2基因表达有关，对研究胃癌分子诊断与治疗具有重要意义。

Inhibitory effect of microRNA-205 on the growth of gastric cancer cell line SGC7901

Objective To explore the effect of miR-205 on the growth of human gastric cancer cell line SGC7901 cells and potential mechanisms.Methods After SGC7901 cells were transfected with miR-205 mimics,the morphological changes of the cells were observed by microscope,the expression levels of miR-205 were determined by qRT-PCR with SYBR Green I,and the expression of ZEB1 and ZEB2 genes were detected by RT-PCR.Results The levels of miR-205 in the cells transfected with miR-205 mimics(0.027 33±0.014) were significantly higher than that of blank group(0.000 619±0.000) and the negative control(0.001 064±0.001),q=5.77 and 5.67,P<0.01.After transfected with miR-205 mim ics,the SGC7901 cells formed fewer colonies and the mRNA levels of ZEB1 and ZEB2 genes were obviously down-regulated.Conclusion miR-205 inhibited the growth of SGC7901 cells by down-regulating the expression of ZEB1 and ZEB2 genes,which may be helpful to investigate the molecular diagnosis and treatment of gastric cancer.