| 恶性胸腹水细胞FCM—DNA定量分析在临床诊断中的应用 |
| |
| 引用本文： | 韩晓芳,冯笑梅,王云峰,阿敏,岱溅勇.恶性胸腹水细胞FCM—DNA定量分析在临床诊断中的应用[J].内蒙古医学杂志,2001,33(4):303-306. |
| |
| 作者姓名： | 韩晓芳 冯笑梅 王云峰 阿敏 岱溅勇 |
| |
| 作者单位： | 1. 内蒙古自治区医院检验科,内蒙古,呼和浩特,010017
2. 大连医学院第二附属医院检验科,辽宁,大连,116027 |
| |
| 基金项目： | 内蒙古自治区自然科学基金 ( 990 30 2 -1 ) |
| |
| 摘 要： | 目的：用流式细胞仪对胸腹腔积液患者同一次胸腹水细胞进行DNA含量测定，并与胸腹水脱落细胞学检查进行对比分析。方法：取10∧6/ml细胞悬液，离心弃上清，加入荧光染料碘化丙啶液，用美国B-D公司的FACS Calibur型流式细胞仪进行测定，用FMu-5型微型玻片离心沉淀法脱落细胞学检查。结果：流式细胞仪检测40例恶性胸腹水细胞DNA非二倍体出现率为90%（36/40），脱落细胞学的检出率为87.5%（35/40）。结论：两种方法联合应用检出率为92.5%（37/40），显著提高良恶性胸腹水的判断率。
|
| 关 键 词： | DNA含量 胸腹水 脱落细胞 流式细胞分析术 FCM 恶性 临床诊断 |
| 文章编号： | 1004-0951(2001)04-0303-03 |
| 修稿时间： | 2001年5月15日 |
Diagnostic evaluation of the FCM-DNA quantitative analysis about the cell of malignant hydrothorax and ascites |
| |
| HAN Xiao-fang,FENG Xiao-mei,WANG Yun-feng,A Min,DAI Jian-yong.Diagnostic evaluation of the FCM-DNA quantitative analysis about the cell of malignant hydrothorax and ascites[J].Inner Mongolia Medical Journal,2001,33(4):303-306. |
| |
| Authors: | HAN Xiao-fang FENG Xiao-mei WANG Yun-feng A Min DAI Jian-yong |
| |
| Affiliation: | HAN Xiao-fang 1,FENG Xiao-mei 1,WANG Yun-feng 1,A Min 1,DAI Jian-yong 2 |
| |
| Abstract: | Objective:The same sample that the cell of hydrothorax and ascites was tested for DNA content by Flow cytometry (FCM),the results were compared with the method of exfoliative cytology.Method:To take 10 6/ml cell suspension,it be centrifugatized and was the supernatant fluid,abandoned the test was performed after we put propidium iodide into it by FACS calibur FCM (made in USA),while exfoliative cytology test was made.Results:The detection rate of DNA non-diploid is 90% by FCM and 87.5% by the method of exfoliative cytology.Conclution:The DNA nondiploid detection rate is 92.5% by use FCM and method of exfoliative extology for compensation.So can more correctivey identify the benign or malignant hydrothorax and ascites. |
| |
| Keywords: | DNA concent Hydrothorax and ascites Exfoliated FCM |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
