天麻素对离体蟾蜍心脏活动影响的实验研究

目的：研究天麻素对离体蟾蜍心率和心脏收缩幅度的影响。方法：采用斯氏法制备离体蟾蜍心脏标本，用BL-410生物机能实验系统观察记录心率和心脏的收缩幅度的变化。结果：天麻素浓度为20μg／ml时，可使离体蟾蜍心率增加（P〉0．05），但是不具有统计学意义；而随着浓度从40ug/ml增加至100ug／ml时，心率明显降低（P〈0．05），有统计学意义。天麻素浓度从20ug／nd增加到100ug／ml时，心脏的收缩幅度明显增加（P〈0．05），有显著差异；但是浓度为100ug／ml时，心脏收缩幅度反而降低，与分别加入60ug／ml、80ug／ml天麻素比较有显著差异，P〈0．01；但是与正常组相比不具有统计学意义P〉0．05。结论：在一定浓度范围内天麻素具有降低心率，增加心脏收缩幅度的作用。

Experimental study of Gastrodin on the isolated toad heart activity

Objective： The research is done to explore the effect of Gastrodin on the heart rate and pulsation strength of isolated toad heart. Methods： We used Schrnow method to make isolated toad heart specimens and BL-410 Biological function experiment system to record the heart rate and the amplitude of contraction. Results： Heart rate increased when the Gastrodin density below 20μg/ ml and reduced as the density added from 40μg/ml to 100μg/ml （ P 〈 0. 05 ） . Amplitude of contraction increased while Gastrodin density added from 40μg/ml to 100μg/ml （P 〈 0. 05） . Amplitude of contraction dropped when Gastrodin density is 100μg/ml com- pared with adding 60ug/ml, 80ug/ml Gastrodin groups （ P 〈 0. 01 ）, but compared with the control group （ P 〉 0. 05 ） . Conclusions： Gastrodin can reduce heart rate and increase amplitude of contraction.