碱性成纤维细胞生长因子对心肌细胞间信号转导的影响

目的 观察碱性成纤维细胞生长因子(FGF-2)对心肌细胞间信号转导的作用。方法 采用激光黏附细胞扫描仪和荧光光淬灭后恢复技术(FRAP)，观察FGF-2作用于心肌细胞1和4天后胞间信号转导的变化。结果 10～100ng／ml的FGF-2能够抑制原代培养的心肌细胞的胞间信号转导，同时促进细胞增生；高浓度的FGF-2(1000ng／ml)使心肌细胞的胞间信号转导明显增加，但抑制细胞生长。结论 FGF-2可以影响心肌细胞的胞间信号转导，可能与其参与心肌细胞的生长、发育甚或凋亡等生物过程有关。

Effects of FGF-2 on the intercellular communication in the cardiac myocytes

Objective To investigate the effects of FGF 2 on the intercellular communication in the cardiac myocytes. Methods The primary neonatal ventricular myocytes of rats were cultured with FGF 2 (0,1,10,100 and 1000ng/ml) for 1 or 4 days. Then the mean fluorescence recovery rate (MFRR, %/min) of these myocytes labeled with carboxyfluocein diacetate after photo bleaching was examined with laser scanning cytopmetry (ACAS Ultima). Results The MFRR levels of the myocytes cultured with FGF 2 (10 and 100ng/ml) groups for 1 day were significantly decreased compared with the control group, while no statistic changes were found among the other groups (FGF 2, 1 and 1000ng/ml) and the control. After FGF 2 exertion on the myocytes for 4 days, the increased cell number, enlarged cellular body and decreased MFRR level were found in the myocytes cultured with FGF 2 (10 and 100ng/ml) groups. And the decreased cell number, disarranged and shrunk cells and significantly increased MFRR level were gotten in the group (FGF 2 1000ng/ml) compared with the control group. Conclusion These results suggested the FGF 2 may have both short and long term effects on the cell cell communication in the cardiac myocytes mediated by gap junction, and be necessary to the cardiac myocyte coordination during the cell growth, differentiation and perhaps apoptosis.