血清对神经嵴细胞向神经胶质细胞分化诱导的体外研究

目的：探讨神经嵴细胞在含血清培养基中向神经细胞的自发分化过程，方法：取妊娠第8．5天的BALB／c胎鼠的神经嵴细胞，分别在无血清和含血清培养基中培养，免疫组化和透射电镜鉴定和观察神经嵴细胞的分化情况.结果：在无血清的培养基中，NSE染色结果阳性，透射电镜下未见神经内分泌颗粒，神经嵴细胞保持其原有的特性，在有血清的培养基中，神经嵴细胞自发分化，胶质纤维酸性蛋白（GFAP），神经特性烯醇化酶（NSE）和S－100染色阳性，透射电镜下可见大量的神经内分泌颗粒。结论：血清可使神经嵴细胞自发的向神经胶质细胞分化。

Induced differentiation of neural crest cells to glial cells in serum medium in vitro

AIM :To study the differentiation of neural crest cells to glial cells in the serum medium. METHODS: Cranial neural tubes were dissected from 8.5th day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells. Neural crest cells were cultured in serum medium and non-serum medium. Characteristics of cells were detected using immunohistochemistry and transmission electron microscopy (TEM) in above two groups. RESULTS : Staining of neuron-specific enolase (NSE) was positive in non-serum medium. In serum medium, neural crest cells were differentiated spontaneously. GFAP?NSE and S-100 were stained positively. Neural endocrine granules were found in TEM. CONCLUSION: Serum can induce neural crest cells differentiating into glial cells spontaeously.