影响小鼠冻融精子体外受精率的主要因素探讨

目的分析影响小鼠精子冷冻成功的因素。方法用含3％脱脂奶和18％棉子糖的冷冻保护剂分别冷冻C57BL／6J、FVB／NJ、B6D2F1、KM和ICR小鼠精子。复苏后，评价小鼠精子的冷冻效果和受精能力。结果各品系小鼠精子冷冻前后的受精率不同；新鲜精子体外受精时，C57BL／6J、FVB／NJ、B6D2F1，KM和ICR五个品系的小鼠的受精率分别为81．4％、87．2％、90．4％、82．7％和79．4％而冻融精子的体外受精率分别为6．7％、46．0％、76．8％、59．2％和31．9％，差异极显著（P〈0．01）。结论遗传背景不同的小鼠品系，精子的受精能力及对冷冻的耐受能力不同。

Exploration of Factors Affecting in vitro Fertilization Rate of Post-Thawed Mouse Spermatozoa

Objective To investigate the factors affecting cryopreservation of mouse sperm.Methods Mice sperm from C57BL/6J,FVB/NJ,B6D2F1 and ICR strains were cryopreserved using 18% raffinose,3% skim milk as cryoprotectant solution,and estimated the survival and fertility of post-thawed sperm from the five strains.The progressive motility of sperms from each strain was affected differently.Results The fertility rate of C57BL/6J,FVB/NJ,B6D2F1,KM and ICR strains were significantly decreased from 81.4%,87.2%,90.4%,82.7% and 79.4%,respectively,of fresh sperm,to 6.7%, 46.0%,76.8%,59.2% and 31.9%,respectively,when using post-thawed sperms(P<0.01).Conclusion The fertility and susceptibility to cryopreservation of sperm from mice of different genetic background differ significantly.