碱性成纤维细胞生长因子对人牙髓细胞增殖和分化的作用

目的 探讨碱笥成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｆａｃｔｏｒ，ｂＦＧＦ）对培养人牙髓细胞增殖和分化的效应。方法 采用四唑盐法、^3Ｈ－ＴｄＲ掺入法、图象分析，检测重组人ｂＦＧＦ（ｈｂＦＧＦ）对细胞ＤＮＡ、胶原蛋白、纤维为连蛋白、碱笥磷酸酶、骨形成蛋白合成和凝集素表达的影响。结果 ｈｂＦＧＦ浓度在１～１０μｇ／Ｌ时显著促进细胞增殖，浓度为１～１００μｇ／Ｌ），Ⅰ型胶原

The effects of basic fibroblast growth factor on proliferation and differentiation of human dental pulp cells in vitro

OBJECTIVE: To evaluate the effects of human basic fibroblast growth factor (hbFGF) on proliferation and differentiation of human dental pulp cells in vitro. METHODS: The syntheses of DNA, collagen, fibronection (FN), alkaline phosphatase (ALP) and bone morphogenetic protein (BMP), and the expression of agglutinin were measured with imagine processing and analysis system. RESULTS: hbFGF at concentration of 1-10 micrograms/L stimulated the cell proliferation measured by MTT colorimetric assay, and promoted incorporation of 3H-thymidine at 1-100 micrograms/L. The expression of type I collagen, FN, Con A receptor, and ALP significantly increased at hbFGF concentration of 10-1,000 micrograms/L, but the expression of type III collagen and WGA receptor markedly decreased at the same concentration. There was no significant difference in expression of BMP. CONCLUSION: bFGF has the capability to promote the differentiation of dental pulp cells to odontoblasts in culture.