北京地区儿童EB病毒感染的血清学调查

目的 了解北京地区儿童EB病毒感染现状.方法 选取北京地区0～14岁儿童的血清589份,应用微润赛润ELISA classic EBV VCA IgG试剂盒,在波长405 nm下检测血清样品的吸光度值.参照试剂盒内专用的标准曲线和临界值判定血清样品EB病毒感染与否.根据专用公式计算EBV VCA IgG抗体活性.利用统计学软件SPSS 13.0分析比较北京城区和农村儿童EBV感染阳性百分率及EBV VCA IgG抗体强度.结果 血清学检测显示北京地区0～14岁儿童EBV感染阳性率为83.6%,其中城市为80.8%,农村为86.2%.EBV感染高峰集中在3岁之前为71%,其中城市为67.7%低于农村75.3%,6岁之前为82.5%.统计学分析比较城市和农村儿童不同年龄的EBV感染阳性百分率和EBV VCA IgG抗体活性具有显著差异.结论 北京城区儿童6岁之前EBV感染阳性百分率有所降低,部分儿童初次感染年龄向后推移.

A serological survey of epstein-barr virus infection in children in Beijing

OBJECTIVE: To understand the prevalence of Epstein-Barr virus (EBV) infection in urban and rural areas of Beijing using the serological method. METHODS: Totally 589 serum samples were collected from children in Beijing urban and rural areas who were 0--14 years old and tested with Viron-Seron ELISA classic EBV virus capsid antigen IgG antibody (EBV VCA IgG) kit. Optical density of serum samples was obtained at the wavelength of 405 nanometers. Sero-positive or negative samples were determined according to standard curve and cut-off attached in ELISA classic EBV VCA IgG kits. The activity of EBV VCA IgG was calculated by using special formula. The percentage and activity of EBV VCA IgG from Beijing children were compared with SPSS 13.0 between the urban and rural areas. RESULTS: The percentage of EBV VCA IgG seropositive samples was 83.6%, and 80.8% in those from urban and 86.2% in those from rural areas. The peak value of EBV infection was 71% seen among children under the age of 3 years, and in urban area the rate was 67.7%, which was lower than that in the rural area (75.3%), and was 82.5% by the age of 6, which was lower than the data (up to 90%) reported 30 years ago. There was a significant difference in EBV infection rate and VCA IgG activities in children at different ages between urban and rural areas (P < 0.05). CONCLUSION: The rate of EBV infection in children living in urban area was lower by the age of 6 years. The primary infection of EBV occurred late in part of children lived in urban area.