儿童末梢血不同放置时间对血常规各参数的影响

目的探讨儿童末梢血不同放置时间对血常规各参数的影响,以此提高临床血常规检测的工作效率。方法收集2015年1月至2016年1月于该院就诊的50名健康儿童的右手无名指末梢血各300μL,室温下(20~25℃)使用全血细胞分析仪于采集即刻(0min),5、10、15、30min进行血常规检验。结果以15min的测定结果为对照,与其他时点的血常规检测结果比较差异无统计学意义(P0.05);与5min的血常规检测结果比较,白细胞计数(WBC)、血小板体积分布宽度(PDW)、淋巴细胞绝对值(LYM)、中性粒细胞绝对值(NEU)、血小板计数(PLT)、红细胞体积分布宽度(RDW)、红细胞压积(HCT)、血小板压积(PCT)差异均具有统计学意义(P0.05)。结论合理安排时间,消除分析前误差,减少末梢血放置时间对血常规各参数的影响,对临床数据的准确判定具有重要意义;建议血常规检测时间控制在10~30min内,以此提高临床检测的工作效率。

Influence of different placing time of child peripheral blood on blood routine parameters

Objective To study the influence of different placing time of child peripheral blood on blood routine parameters in order to increase the working efficiency of clinical blood routine test.Methods Each 300 μL of peripheral blood was collected from the right hand ring finger in 50 healthy children in our hospital from January 2015 to January 2016.The blood routine was performed by using the whole blood cells analyzer at instantly after blood collection,at 0,5,10,15,30 min after blood collection under the room temperature (20 to 25 ℃).Results With the measured results at 15 min as the control,the detection results at the other times points had no statistical difference (P>0.05).Compared with the detection results at 5 min,the white blood cell count(WBC) and platelet volume distribution width (PDW),lymphocyte absolute value (LYM),neutrophil percentage absolute value (NEU),platelet count (PLT),red blood cell volume distribution width (RDW),hemotocrit (HCT) and plateletcrit(PCT) were statistically different (P<0.05).Conclusion Reasonably arranging time,eliminating pre-analysis error and reducing the influence of peripheral blood placing time on blood routine parameters have an important significance to accurately judge the clinical dat.It is recommended that the blood routine detection time should be controlled within 10-30 min in order to increase the working efficiency of clinical detections.