广州地区儿童肺炎支原体核酸检测阳性病例的分析

目的分析该地区儿童肺炎支原体核酸(MP-DNA)检测阳性病例的临床特征,为临床诊断和治疗儿童肺炎提供依据。方法对广州医学院荔湾医院和广州医科大学附属第一医院2014-2015年门诊及住院患者中有呼吸道感染症状者,进行咽拭子采样,提取DNA后用聚合酶链反应法检测MP基因,同时抽血检测血常规和肺炎支原体抗体。结果 2 018例呼吸道感染患儿中MP-DNA阳性209例,阳性率10.36%。3~14岁年龄组MP-DNA阳性率为18.25%,均高于其他年龄组,差异具有统计学意义(P0.05);7-9月份阳性率为16.11%,均高于其他月份组,差异具有统计学意义(P0.05);女性阳性率为13.04%,明显高于男性的8.79%,差异具有统计学意义(P0.05);MP-DNA阳性患者白细胞计数偏高占22.01%,白细胞计数正常内占49.28%,白细胞计数偏低占28.71%;MP-DNA阳性患者血清MP-IgG检测中阳性146例,占69.86%。结论 MP-DNA检测对小儿呼吸道感染诊断有重要价值,进行多项目及时快速地检测,对预防该疾病加重有重要意义。

Analysis of mycoplasma pneumoniae nucleic acid testing positive cases of children in Guangzhou

Objective To analysis the clinical features of mycoplasma pneumoniae nucleic acid(MP-DNA)testing positive cases of from the region's children,provide the basis for clinical diagnosis and treatment.Methods Outpatient and hospitalized patients with respiratory infection symptoms were sampled pharyngeal swab in the region from 2014 to 2015.After DNA was extracted,polymerase chain reaction method was conducted to detect the MP gene.At the same time,blood routine and mycoplasma pneumoniae antibody were detected from drawing blood.Results There were 209 cases of MP-DNA positive from 2018 cases in children with respiratory tract infection,the positive rate was 10.36 ％.MP-DNA positive rate in 3-＜ 14 years old age group was 18.25 ％,which was higher than other age groups(P＜0.05).The positive rate was 16.11％ from July to September,which was higher than in other month groups(P＜0.05).The positive rate of girl (13.04 ％) was significantly higher than that of boy (8.79 ％) (P＜ 0.05).WBC count was higher than normal for 22.01 ％,normal accounted for 49.28 ％,lower than normal for 28.71％ in MP-DNA positive children.Serum MP-IgG positive rate was 69.86％ in MP-DNA positive children.Conclusion MP-DNA testing has important value to the pediatric respiratory infection diagnosis,detection of multiple projects has important significance to preventing illness aggravating.