肿瘤型M2-PK、APC、K-ras检测在结直肠癌诊断中的意义

目的检测结直肠癌患者粪便和血清的M2型丙酮酸激酶(M2-PK)、APC、K-ras水平,并分析M2-PK、APC、K-ras的水平与结直肠癌病理T分期的关系。方法将2015年1-10月在该院手术治疗的结直肠癌患者200例纳入该研究作为患者组,另外选取健康体检者100例作为对照组。采用酶联免疫吸附测定(ELISA)检测结直肠癌患者血清和粪便的M2-PK、APC、Ras水平,并结合患者的病理资料对M2-PK、APC、K-ras的水平与结直肠癌病理T分期的关系进行分析。结果结直肠癌患者血清和粪便的M2-PK、APC、K-ras的表达水平分别高于对照组(P0.05)。M2-PK、APC、K-ras的表达与结直肠癌的病理T分期有密切关系,且临床病理T分期较晚的患者M2-PK、APC、K-ras的表达水平更高(P0.05)。结论结直肠癌患者血清和粪便的M2-PK、APC、K-ras水平较高且与肿瘤的临床病理T分期有关。M2-PK、APC、K-ras可作为早期诊断结直肠癌的分子标志物。

Clinical significance of combined detection of fecal tumor M2-PK,APC ,K-ras expression in early diagnosing colorectal cancer

Objective To detect the expression levels of fecal tumor M 2-pyruvate kinase (M2-PK ) ,APC and K-ras in the pa-tients with colorectal cancer (CRC) and to analyze the relationship between the expression change of M 2-PK ,APC and K-ras with the CRC pathologic stages .Methods The feces samples in 100 patients with CRC operatively treated in our hospital from January to October 2015 were selected as the research objects and which in 100 persons undergoing physical emination served as the control group .The serum and fecal M2-PK ,APC and K-ras expression levels were detected by using enzyme-linked immunosorbent assay (ELISA) .Furthermore the relationship between the M2-PK ,APC and K-ras expression levels with CRC pathologic stages was ana-lyzed by combining with their pathological data .Results The expression levels of serum and fecal M2-PK ,APC and K-ras in the patients with CRC were significantly higher than those in the control group (P<0 .05) .Meanwhile the above results showed that the expression of M2-PK ,APC and K-ras had a close relationship with the pathological T stage ,moreover the later the pathological stage ,the higher the levels of M2-PK ,APC and K-ras ,the difference among different stages had statistical significance(P<0 .05) . Conclusion Serum and fecal M2-PK ,APC and K-ras are highly expressed in serum and feces ,and which are closely related with the clinicopathological stages .M2-PK ,APC and K-ras may be used as the novel molecular markers for early diagnosis of CRC .