| RT-PCR检测在疑似肺炎支原体感染患者中的应用研究 |
| |
| 引用本文： | 胡淼,马燕芬,李向平,马亚琼,李平法. RT-PCR检测在疑似肺炎支原体感染患者中的应用研究[J]. 国际检验医学杂志, 2017, 38(11). DOI: 10.3969/j.issn.1673-4130.2017.11.014 |
| |
| 作者姓名： | 胡淼 马燕芬 李向平 马亚琼 李平法 |
| |
| 作者单位： | 1. 新乡医学院三全学院,河南新乡,453003;2. 西安交通大学第一附属医院,西安,710061;3. 河南省骨科医院,郑州,450000;4. 汝州市人民医院,河南平顶山,467500;5. 新乡医学院,河南新乡,453003 |
| |
| 摘 要： | 目的探讨实时荧光定量聚合酶链反应(PCR)检测肺炎支原体(MP)DNA在疑似MP感染患者中的应用价值。方法采用RT-PCR对疑似MP感染患者血清、痰、胸腔积液、鼻咽拭子、肺泡灌洗液、支气管灌洗液标本进行MP-DNA检测。结果 1 402例标本MP-DNA检测总阳性率为12.20%,血清标本检测阳性率最低,仅为2.36%,痰、肺泡灌洗液及支气管灌洗液检测阳性率较高,依次为62.96%、77.08%和88.71%。结论 RT-PCR法适用于多种类型标本MP-DNA检测,是诊断MP感染的有效方法之一。
|
| 关 键 词： | 实时荧光定量 聚合酶链反应 肺炎支原体 DNA |
Application value of RT-PCR in Mycoplasma pneumoniae infection |
| |
| HU Miao,MA Yanfen,LI Xiangping,MA Yaqiong,LI Pingfa. Application value of RT-PCR in Mycoplasma pneumoniae infection[J]. International Journal of Laboratory Medicine, 2017, 38(11). DOI: 10.3969/j.issn.1673-4130.2017.11.014 |
| |
| Authors: | HU Miao MA Yanfen LI Xiangping MA Yaqiong LI Pingfa |
| |
| Abstract: | Objective To investigate the clinic significance of real-time fluorescent quantity polymerase chain reaction(RT-PCR) for detection of Mycoplasma pneumoniae(MP) DNA in patients suspected with MP infection.Methods A total of 1 402 samples,including serum,sputum,pleural fluid,nasopharyngeal swab,alveolar irrigating solution and bronchial irrigating solution,were detected for MP-DNA by using RT-PCR.Results The total positive rate all samples were 12.20%.The positive rate of serum was the lowest,which was 2.36%.The positive rates of sputum,alveolar irrigating solution and bronchial irrigating solutions were relatively high,which were 62.96%,77.08% and 88.71%,respectively.Conclusion RT-PCR could be fitted for the detection of MP-DAN in various samples,which could be effective method for the diagnosis of MP infection. |
| |
| Keywords: | real-time fluorescent quantity polymerase chain reaction Mycoplasma pneumoniae DNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
