免疫性血小板减少症患者P-糖蛋白与淋巴细胞亚群的相关性研究

目的探讨免疫性血小板减少症患者P-糖蛋白(P-gp)与淋巴细胞亚群(CD4~+、CD8~+)的相关性研究。方法选取2015年8月至2016年9月该院确诊的免疫性血小板减少症患者20例,依据血小板计数分为轻度组和重度组,同期该院体检中心健康体检者20例纳入对照组,所有研究对象均采用流式细胞技术检测血浆淋巴细胞亚群(CD4~+、CD8~+)和P-gp水平,采用Pearson相关分析法分析血浆P-gp与血浆CD4~+、CD8~+、CD4~+/CD8~+的关系,统计分析所有研究对象血浆血小板计数、淋巴细胞亚群和P-gp水平。结果重度组患者血浆CD4~+、CD8~+、CD4~+/CD8~+和P-gp水平明显高于轻度组,差异有统计学意义(t=3.587、2.957、2.315、2.646,P0.05),轻度组患者血浆CD4~+、CD8~+、CD4~+/CD8~+和P-gp水平明显高于对照组,差异有统计学意义(t=5.479、2.921、4.536、7.750,P0.05)。血浆P-gp水平与血浆CD4~+、CD8~+、CD4~+/CD8~+水平呈正相关(r=0.683、0.617、0.548,P0.05)。结论血浆CD4~+、CD8~+、CD4~+/CD8~+和P-gp水平与免疫性血小板减少症患者病情有关,血浆P-gp水平与患者免疫功能异常亢进存在密切联系,提示检测其水平变化有助于医师评估患者药物治疗疗效。

Correlation studies of P-glycoprotein and lymphocyte subgroup in patients with immune thrombocytopenia

Objective To discuss the correlation of P-glycoprotein(P-gp) and lymphocyte subgroup(CD4+,CD8+) in patients with immune thrombocytopenia.Methods A total of 20 patients with immune thrombocytopenia were selected from August 2015 to September 2016 in the Eighth Hospital Affiliated to Sun Yat-sen University.According to the platelet count,all patients were divided into mild group and severe group,20 cases of health persons were selected at the same period as the healthy group,flow cytometry technique was used to detect the plasma lymphocyte subsets(CD4+,CD8+) and P-gp level of all the objects.Pearson correlation analysis was used to analyze the relationship between plasma P-gp and plasma CD4+,CD8+,CD4+ / CD8+ level,analyzed all the objects plasma platelet count,P-gp and CD4+,CD8+,CD4+ / CD8+ levels.Results Plasma CD4+,CD8+,CD4+ / CD8+ and P-gp level of severe group were significant higher than those of mild group(t=3.587,2.957,2.315,2.646,P<0.05),plasma CD4+,CD8+,CD4+ / CD8+ and P-gp level of mild group patients were significant higher than those of healthy group(t=5.479,2.921,4.536,7.750,P<0.05).Pearson correlation analysis results showed that the plasma levels of P-gp and plasma CD4+,CD8+,CD4+ / CD8+ levels were positively correlated(r=0.683,0.617,0.548,P<0.05).Conclusion Plasma CD4+,CD8+,CD4+/CD8+and P-gp level is associated with immune thrombocytopenia patients condition,plasma levels of P-gp closely links with abnormal immune function in patients with hyperthyroidism,prompting detection the level of change could help doctors to assess patients medication curative effect.