血浆总tau蛋白和磷酸化tau蛋白在阿尔茨海默病中的诊断意义

目的探讨血浆总tau(T-tau)蛋白和磷酸化tau(P-tau)蛋白在阿尔茨海默病(AD)中的诊断意义。方法以天津市北辰区某社区服务中心≥60岁的22 352例体检者作为调查对象,运用随机整群抽样方法,先采用简易智力状态检查量表(MMSE)和日常生活能力评定表(ADL)进行认知功能和日常能力的评分,评分低于标准者再使用Hamilton抑郁量表和Hachinski缺血量表进行诊断性评分,相关的实验室检查排除其他中枢神经系统,以及其他系统和物质原因等所致的痴呆患者,最终由神经内科医师确诊AD患者105例,将无中枢神经系统疾病、无痴呆的非神经系统疾病患者作为健康对照组,共42例。采用ELISA方法检测两组受试者的血浆T-tau蛋白和P-tau蛋白水平。结果 AD组和健康对照组血浆T-tau和P-tau蛋白水平比较,AD组血浆T-tau水平(15.93±6.59)ng/L高于健康对照组(14.10±6.32)ng/L,但差异无统计学意义(P0.05);AD组血浆P-tau蛋白水平(1.26±0.75)ng/L高于健康对照组(0.69±0.24)ng/L,差异有统计学意义(P0.05)。结论血浆磷酸化tau蛋白水平可能对AD患者具有辅助诊断价值。

Diagnostic significance of plasma total tau and phosphorylated tau protein in Alzheimer's disease

Objective To investigate the plasma total tau(T-tau)and phosphorylated protein tau(P-tau)protein in the diagnosis of Alzheimer's disease(AD).Methods 22 352 medical patients aged over 60 years in Beichen Area Community Service Center were involved in this survey.Random sampling principle was used for screening.Mini-mental state examination(MMSE)and activities of daily living assessment form(ADL)were conducted for the cognitive function and ability of daily score firstly.When the score below the standard,the Hamilton depression scale and Hachinski ischemic scale were re-used for diagnostic score.Related laboratory tests were conducted to exclude other central nervous system and other systems and material causes of dementia patients.Ultimately,105 cases of AD,diagnosed by the neurologist,and 42 cases of non central nervous system disease,non dementia non nervous system disease patients as the normal control group were involved.T-tau and P-tau levels in the two groups were determined by ELISA.Results the T-tau concentration in AD group(15.93+6.59)ng/L was higher than control group(14.10±6.32)ng/L,no significant difference was found (P ＞ 0.05).However,compared with control group (0.69 ± 0.24) ng/L,P-tau protein in AD group (1.26 ± 0.75)ng/L increased significantly(P＜0.05).Conclusion Plasma levels of phosphorylated tau protein might have diagnostic value for AD patients.