| CTP-OD1-HA和CTP-OD2-HA融合肽或联合imatinib对K562细胞增殖的影响 |
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| 引用本文： | 肖恒,任彦斌,杨志明,周淑杰,尹蕾,秦志梅,许玲,李守霞. CTP-OD1-HA和CTP-OD2-HA融合肽或联合imatinib对K562细胞增殖的影响[J]. 国际检验医学杂志, 2017, 38(14). DOI: 10.3969/j.issn.1673-4130.2017.14.002 |
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| 作者姓名： | 肖恒 任彦斌 杨志明 周淑杰 尹蕾 秦志梅 许玲 李守霞 |
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| 作者单位： | 1. 河北省邯郸市中心医院检验科 056000;2. 河北省邯郸市中心医院功能科 056000 |
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| 摘 要： | 目的研究CTP-OD1-HA和CTP-OD2-HA融合肽及联用imatinib对K562细胞增殖的影响。方法将前期制备的CTP-OD1-HA和CTP-OD2-HA融合肽或连同imatinib作用于K562细胞后,应用细胞增殖及细胞毒性检测试剂盒(MTT)、克隆形成试验方法检测和比较细胞增殖情况。结果 MTT检测发现,CTP-OD1-HA和CTP-OD2-HA融合肽能够抑制K562细胞增殖,联用imatinib后,效果更加明显;克隆形成试验结果显示,CTP-OD1-HA和CTP-OD2-HA可抑制K562细胞株的克隆形成能力。结论 CTP-OD1-HA和CTP-OD2-HA融合肽可以特异性抑制K562细胞的增殖,并增加imatinib的敏感性。
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| 关 键 词： | 慢性粒细胞白血病 融合肽 细胞增殖 BCR-ABL |
The influence of CTP-OD1-HA and CTP-OD2-HA fusion peptides or combine with imatinib on proliferation of K562 cells |
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| XIAO Heng,REN Yanbin,YANG Zhiming,ZHOU Shujie,YIN Lei,QIN Zhimei,XU Ling,LI Shouxia. The influence of CTP-OD1-HA and CTP-OD2-HA fusion peptides or combine with imatinib on proliferation of K562 cells[J]. International Journal of Laboratory Medicine, 2017, 38(14). DOI: 10.3969/j.issn.1673-4130.2017.14.002 |
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| Authors: | XIAO Heng REN Yanbin YANG Zhiming ZHOU Shujie YIN Lei QIN Zhimei XU Ling LI Shouxia |
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| Abstract: | Objective To study the influence of CTP-OD1-HA and CTP-OD2-HA fusion peptides and combined with imatinib on proliferation of K562 cells.Methods K562 cells were treated with CTP-OD1-HA and CTP-OD2-HA peptides or together with imatinib.The proliferation of cells were detected and compared by MTT and clone formation methods.Results MTT examination demonstrated that CTP-OD1-HA and CTP-OD2-HA peptides could inhibit the proliferation of K562 cells,and the effect was more obvious when acted along with imatinib;Clone formation showed that CTP-OD1-HA and CTP-OD2-HA peptides suppressed the continuous colony forming ability of K562 cells.Conclusion CTP-OD1-HA and CTP-OD2-HA could specially inhibit the proliferation of K562 cells,and increase the sensitivity of imatinib. |
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| Keywords: | chronic myeloid leukemia fusion peptide cell proliferation BCR-ABL |
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