干扰化学发光法检测梅毒螺旋体抗体的疾病分析

目的探讨若干疾病在使用化学发光法检测梅毒螺旋体抗体时的干扰情况。方法收集110例9种感染不同疾病的标本和200例临床随机标本,共310例标本使用雅培ARCHITECT i2000全自动化学发光免疫分析仪及其配套试剂和罗氏e602全自动化学发光免疫分析仪及其配套试剂同时检测,结果不一致的标本使用明胶颗粒凝集试验(TPPA)进行确认。结果两台仪器检测结果不一致的标本共有5例,TPPA确认5例标本均为阴性,4例均为多发性骨髓瘤患者标本。结论(1)雅培ARCHITECT i2000和罗氏e602检测梅毒螺旋体抗体一致性较好;(2)多发性骨髓瘤患者在使用化学发光法检测梅毒螺旋体抗体时可能存在干扰,而造成假阳性结果,日常检验工作需警惕。

Analysis on diseases interfering treponema pallidum antibody detection by chemiluminescence

Objective To explore the interference situation of several diseases in the detection of treponema pallidum antibody by chemiluminescence method.Methods One hundred and ten samples of 9 kinds of different infectious diseases and 200 clinical random samples were collected.Three hundreds and ten samples were simultaneously detected by using the Abbott ARCHITECT i2000 automatic chemiluminescence immunoassay analyzer and matched reagents,and the Roche E602 automatic chemiluminescence instrument and its matching reagent.The samples of inconsistent results were confirmed by the gelatin particle agglutination test (TPPA).Results There were 5 samples with inconsistent results detected by two instruments,5 samples confirmed by TPPA were negative,and 4 cases were the samples of multiple myelome.Conclusion (1)Abbott ARCHITECT i2000 and Roche E602 have a good consistency in detecting treponema pallidum antibody;(2)for the patients of multiple myeloma,the interference may exist in detecting treponema pallidum antibody by chemiluminescence method,which may cause false positive results and needs to be vigilant in our daily work.