儿童血浆和尿液中性粒细胞明胶酶相关脂质运载蛋白正常参考值的建立

目的建立上海某儿童医院血浆和尿液中性粒细胞明胶酶相关脂质运载蛋白(NGAL)正常参考值。方法选取2014年5-10月上海交通大学附属儿童医院健康儿童新鲜EDTA抗凝血浆183份和新鲜尿液125份,检测NGAL水平。根据CLSI C28-A2按年龄分组,取x±1.96s建立正常参考值。结果各年龄组之间正常参考值范围间差异具有统计学意义(P0.05)。健康儿童血浆NGAL正常参考值:0~7个月,291.28μg/L;7个月至5岁,150.87μg/L;5~9岁,127.93μg/L;9~≤16岁,161.74μg/L;健康儿童尿液NGAL正常参考值:0~7个月,257.31μg/L;7个月至5岁,201.55μg/L;5~9岁,197.69μg/L;9~≤16岁,151.46μg/L。0~7个月组血浆和尿液NGAL结果较其他3组高。结论建立儿童医院血浆和尿液NGAL的正常参考值,为儿科急性肾功能损伤的诊断和治疗提供临床依据。

Establishment of normal reference intervals of plasma and urine neutrophil gelatinase associated lipocalin in children

Objective To establish normal reference intervals of plasma and urine neutrophil gelatinase-associated lipocalin (NGAL) in children′s hospital.Methods A total of 183 fresh EDTA anticoagulant samples and 125 fresh urine in healthy children were collected from May 2014 to October 2014.According to the CLSI C28-A2 ,the unilateral upper limit 95% was established the normal reference value in different age group.Results There was significant difference in four groups (P<0.05).The normal reference intervals of plasma NGAL in healthy children:0 to <7 months;<291.28 μg/L;7 months to <5 years old;<150.87 μg/L;5 years old to <9 years old:<127.93 μg/L;9 years old to ≤16 years old:<161.74 μg/L;the normal reference intervals of healthy children urine NGAL:0 to <7 months:<257.31 μg/L;7 months to <5 years old:<201.55 μg/L;5years old to <9 years old:<197.69 μg/L;9 years old to ≤16 years old:<151.46 μg/L.Plasma and urine NGAL results in neonatal group were higher than the other three groups.Conclusion The normal reference intervals of plasma and urine NGAL in children′s hospital is established.this could provide clinical evidence for the diagnosis and treatment of acute renal injury in pediatric patients.