改良琼脂糖电泳法测定BALP在前列腺癌骨转移诊断中的应用

目的探讨改良琼脂糖电泳法检测骨碱性磷酸酶同工酶(BALP)在前列腺癌(PCa)骨转移诊断中的应用。方法神经氨酸苷酶处理血清,采用改良的琼脂糖电泳法分离测定50例健康体检者和163例PCa患者血清中的ALP同工酶。结果 (1)普通的琼脂糖电泳法对肝和BALP的分辨率较低,改良琼脂糖电泳法提高了分辨率。(2)PCa组的总ALP和BALP均高于对照组,PCa骨转移组的总ALP和BALP活性均显著高于对照组和无骨转移组(P0.05),PCa无骨转移组的总ALP和BALP活性和对照组差异无统计学意义(P0.05)。结论 BALP与PCa骨转移存在一定的相关性,经神经氨酸苷酶处理血清后,采用改良琼脂糖电泳法分离ALP同工酶有助于临床辅助诊断PCa有无骨转移。

Application of modified agarose electrophoresis for detecting bone alkaline phosphatase isoenzyme in diagnosis of bone metastasisof prostate carcinoma

Objective To discuss the application of improved agarose electrophoresis for detecting bone alkaline phosphatase(BALP) isoenzymes in the diagnosis of metastasis in prostate carcinoma(PCa).Methods Serum ALP isoenzymes in 50 persons undergoing physical examination and 163 cases of PCa were detected by the improved agarose electrophoresis after treating serum by neuraminidase.Results (1)The resolution of liver and bone ALP by the common agarose gel electrophoresis was lower,and which was increased by the modified agarose electrophoresis.(2)Total ALP and BALP in the PCa group were higher than those in the control group;total ALP and BALP activity in the PCa bone metastasis group were higher than those in the control group and non-metastasis group(P<0.05),there were no statistical difference in BALP and total ALP between the non-bone metastasis group and control group(P>0.05).BALP and total ALP in bone metastasis of prostate carcinoma were higher than no bone metastasis.Conclusion Bone ALP has a certain correlation with PCa bone metastasis,also between bone ALP and bone metastasis of carcinoma.After treating serum by neuraminidase,adopting the improved agarose gel electrophoresis for separating ALP isozymes contributes to the clinical auxiliary diagnosis of PCa bone metastasis.