精浆和血清抗苗勒管激素与精液参数的相关性研究

目的探讨精浆和血清抗苗勒管激素(AMH)水平与男性精子数量和活力的相关性。方法选取到广东省中山市博爱医院生殖门诊就诊的、因女性原因不孕的健康男性215例,检测精浆、血清AMH及精液参数(精子的密度、活率、活力及畸形率),测定血清性激素6项。分别以精浆和血清AMH为因变量,采用多重线性回归模型探讨其与精液参数和性激素水平的定量关系。结果 215例研究对象中,总精浆AMH中位数为0.47,四分位数为0.05~3.09pmol/次射精。血清AMH中位数为53.07,四分位数为32.32~72.20pmol/L。经过多重线性回归分析,在矫正年龄和体质量指数后,精浆AMH与精子总数、精液浓度、前向精子活动力、总精子活动力、血清抑制素B呈正相关(P0.05);与精子形态和其他血清性激素相关性比较,差异无统计学意义(P0.05);血清AMH与血清促卵泡激素呈负相关,与血清抑制素B呈正相关(P0.05);与精浆各参数和其他血清性激素相关性比较,差异无统计学意义(P0.05)。结论精浆AMH个体差异大,与精液浓度、精子总数、精子活力呈正相关,尚未发现血清AMH与其相关。

Anti-mullerian hormone in seminal plasma and serum:association with sperm parameters

Objective To explore the association of anti-mullerian hormone(AMH)in seminal plasma and serum with sperm counts and energy for male.Methods For 215 cases of healthy male selected from our reproductive clinic,with women′s reason for infertility,seminal plasma and serum AMH were detected,as semen parameters(sperm density,living rate,vitality and malformation rate),6 items of serum sex hormone.In seminal plasma and serum AMH respectively as the dependent variable,using multiple line-ar regression model to explore its quantitative relation with semen parameters and sex hormone levels.Results 215 cases were en-rolled,aged 34.28±5.70 years,while the median of the seminal plasma AMH was 0.47,quartile 0.05-3.09 pmol/ejaculation.The median of the serum AMH was 53.07,quartile 32.32 -72.20 pmol/L.Through multiple linear regression analysis,after adjusted by age and BMI,the seminal plasma of AMH and total number of sperm,sperm concentration,dynamic motility,total sperm activi-ty,serum inhibin B were positively correlated(P <0.05);The correlation between sperm morphology and other serum sex hormone had no statistical significance(P >0.05);Serum AMH negatively correlated with serum FSH,with serum inhibin B positively(P <0.05);Seminal plasma in various parameters and other related serum sex hormone had no statistical significance(P >0.05).Conclu-sion The seminal plasma of AMH were positively correlated with sperm concentration,sperm counts,sperm vitality,with the asso-ciation for serum AMH not yet found.