胎儿性RNA结合蛋白p62在肝细胞癌中表达的意义

目的　探讨表达p6 2的肝癌组织的病理形态学特点 ,以及 p6 2与癌细胞增殖和转移等生物学特性的关系。方法　用免疫组织化学方法检测 4 0例原发性肝细胞肝癌 p6 2和Ki 6 7的表达。结果　p6 2阳性率是 6 7.5 % (2 7/ 4 0 ) ,其中 p6 2强阳性表达的 12例中 9例为肝癌病理学分级为Ⅲ级的病例。p6 2表达强阳性的癌组织往往间质有大量的纤维组织或有大片坏死癌细胞 ,其增殖活性也高 ,Ki 6 7标记指数为 2 8 3%± 15 1% ,与 p6 2表达阴性组 (7 5 %± 9 8% )比较 ,差异具有显著性意义 (t=3 0 87,P =0 0 0 5 ) ;5例有肺转移或腹腔淋巴结转移的病例 ,其中 3例p6 2表达强阳性。结论　p6 2的表达与癌细胞的增殖和转移有一定的关系 ,和间质纤维组织较多的微环境有关

Significance of a novel fetal RNA-binding protein p62 expression in hepatocellular carcinoma

OBJECTIVE: To investigate the histological characteristics of hepatocellular carcinoma with p62 expression, and the correlation of p62 protein with biological characteristics of the cancer cells such as proliferation and metastasis. METHODS: The immunohistochemical expression of p62 and Ki-67 were studied in 40 primary hepatocellular carcinomas. RESULTS: 67.5% (27/40) exhibited positive staining of p62 protein in the cytoplasm of malignant cells, of which the strong positivity was mainly found in pathologic grade III cases. The cases with strong positive staining of p62 exhibited extensive fibrosis in the stroma and massive necrosis of the tumor. These cases also exhibited high proliferation activity, the mean Ki-67 labeling index was 28.3% +/- 15.1%, which was significantly higher than that in p62 negative ones (t = 3.087, P = 0.005). Three of five cases with lung or celiac lymph node metastasis exhibited strong positive staining of p62. CONCLUSION: There is some relationship between the expression of p62 protein, proliferation activity and metastasis of cancer cells as well as with the microenvironment which have abundant fibrous tissue in the stroma.