| 异黄酮活性成分对Aβ_(1-42)诱导的PC12细胞损伤的保护作用 |
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| 引用本文： | 杨红,赵宇红,陈伟强,罗少洪,金桂芳,潘伟. 异黄酮活性成分对Aβ_(1-42)诱导的PC12细胞损伤的保护作用[J]. 中国药理学通报, 2005, 21(9): 1089-1091 |
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| 作者姓名： | 杨红 赵宇红 陈伟强 罗少洪 金桂芳 潘伟 |
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| 作者单位： | 1. 广东药学院,基础学院生物化学教研室,广东,广州,510224
2. 广东药学院,药理学教研室,广东,广州,510224 |
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| 基金项目： | 广东省科技厅科技计划，广东省中医药管理局科研项目 |
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| 摘 要： | 目的探讨异黄酮活性成分对β淀粉样蛋白(Aβ1-42)诱导的PC12细胞损伤的保护作用。方法从大豆中提取纯化异黄酮活性成分,以Aβ1-42诱导PC12细胞损伤为细胞模型,采用细胞形态学方法、MTT法、LDH法观察不同剂量的异黄酮活性成分对PC12细胞损伤的保护作用。结果异黄酮活性成分能有效地改善PC12细胞的形态,提高细胞存活率(P<0.05),保护组LDH活性低于损伤组(P<0.05)。结论大豆异黄酮活性成分对Aβ1-42诱导的PC12细胞损伤具有显著的保护作用。
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| 关 键 词： | 异黄酮活性成分 β淀粉样蛋白 PC12细胞 |
| 文章编号： | 1001-1978（2005）09-1089-03 |
| 收稿时间： | 2005-01-04 |
| 修稿时间： | 2005-03-22 |
The protective effect of active components of isoflavones on the PC12 cells damage induced by Aβ1-42 |
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| YANG Hong,ZHAO Yu-hong,CHEN Wei-qiang,LUO Shao-hong,JIN Gui-fang,PAN Wei. The protective effect of active components of isoflavones on the PC12 cells damage induced by Aβ1-42[J]. Chinese Pharmacological Bulletin, 2005, 21(9): 1089-1091 |
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| Authors: | YANG Hong ZHAO Yu-hong CHEN Wei-qiang LUO Shao-hong JIN Gui-fang PAN Wei |
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| Abstract: | Aim To study the effect of active components of isoflavones on PC12 cells damage induced with Aβ_(1-42). Methods The active components of isoflavones were extracted. Using PC12 cells damage induced by Aβ_(1-42) as the model,the protective effect of active components was determined with morphological examination and MTT and LDH assays. Results The morphological change of the damaged PC12 cells was improved, and the survival rate of the cells was significantly increased(P<0.05).The LDH activity in the protective group was markedly decreased (P<0.05). Conclusion The protective effect of active components of isoflavones was significant on PC12 cells damage induced by Aβ_(1-42). |
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| Keywords: | active components of isoflavones amyloidbeta-protein PC 12 cells |
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