MMP-2及其抑制剂在子宫内膜异位症中的表达及与临床分期的关系

目的　研究基质金属蛋白酶 2 (MMP 2 )及其抑制剂 (TIMP 2 )在子宫内膜异位症 (EMs)中的表达 ,探讨其在EMs发生发展中的作用。方法　应用免疫组化S P法检测 50例异位内膜组织标本 ,2 0例在位内膜组织中MMP 2 ,TIMP 2蛋白的表达情况。结果　MMP 2在 50例异位内膜组织中有表达者 41例 ,阳性表达率为 82 % ,而在 2 0例在位内膜组织中 ,有表达者 2例 ,阳性表达率为 1 0 % ,两组比较差异有极显著意义 (P <0 0 1 )。检测同时发现 ,MMP 2随EMs临床分期增高表达上调 ,TIMP 2则随临床分期增高表达下调 ,两者比较差异具有统计学意义 (P <0 0 5)。结论　MMP 2表达增强和TIMP 2表达下降 ,可能在EMs发生发展中发挥关键作用 ,两者表达的平衡失调程度可作为评估EMs进展及预后不良的指标。

Study on the relationship between the clinical stage and expression of MMPs and TIMPs in endometriosis

Objective To study the expression of ma tr ix metalloproteinase-2(MMP-2)and tissue inhibitor of metalloproteinase-2(T IMP-2)in endometriosis (EMs) and to discuss the role in the occurrence and dev elopment of EMs. Methods Immunohistochemistry S-P meth od was employed to detect the expression of MMP-2 and TIMP-2 in the ectopic en dometrium of the patients(50 cases), the eutopic endometrium of the patients w ith endometriosis(20 cases). Results The positive exp ressions of MMP-2 in the ectopic endometrium was 41 in 50 cases of the patients with endometriosis, the positive expression rate was 82%. But the expressions o f MMP-2 in the eutopic endometrium of 2 in 20 cases were positive; the positive expression rate was 10%. There was extremely difference between the two groups (P< 0.01). The expression of MMP-2 was up-regulated with the increasing cl inical stage of endometriosis, but the expression of TIMP-1 was down-regulated with the increasing clinical stage. The difference has statistic significance i n MMP-2 and TIMP-2(P<0.05). Conclusions The inc reasing expression of MMP-2 and the decreasing expression of TIMP-2 may be key role in occurrence and development of EMs, and the unbalanced expression degree could act as an index to evaluate the progress and prognosis in endometriosis.