实验性肝损伤模型建立的初步探讨

目的建立四氯化碳(CCl4)所致大鼠急性肝损伤、小鼠四氯化碳反复给予造成肝中毒及酒精性肝病小鼠模型,筛选最佳给药剂量和次数。方法①SD大鼠每隔2天给予CCl41次,连续4次(10天),采用灌胃(ig)和腹腔注射(ip)的两种途径,测定谷丙转氨酶(ALT)及谷草转氨酶(AST),并测体重和肝脏重、胸腺重,求脏器系数。②给小鼠CCl4灌胃给药,观察CCl4给药后24-96h(1-4d)的血清ALT变化。③给小鼠不同剂量、次数和时间的酒精,测定血清ALT。结果①CCl4所致大鼠急性肝损伤模型给药途径以腹腔注射为好,CCl4浓度以10%为好。②小鼠四氯化碳反复给予造成肝中毒,以20%CCl40.05ml/10g,三天1次灌胃给药的方法为好。③关于酒精所致小鼠急性肝损伤模型,以二锅头酒原液0.1ml/10g一天1次,5-7天的酒量为好。结论本试验筛选了实验性肝损伤模型的CCl4浓度、剂量及给药途径和酒精剂量、次数和时间,但为更好地建立模型,有待进一步探讨。

Preliminary Exploration on Experimental Models of Liver Damage

Objective: To set up the acute liver damage model in rats and the liver intoxication by using CCl4 in mice as well as the model of alcoholic liver disease in mice, and then to explore the best dose and times of treatment for them. Methods: ① The SD rats were given CCl4 once every three days by intraperitoneal and intragastrical injection. After four times, the levels of ALT and AST in serum were determined and the body weight, liver weight and thymus weight of each rat were measured. ②The mice were given CCl4 by intragastrical injection, and then the observation was made to see the change of serum ALT within 1 to 4 days. ③ The mice were given alcohol with different dose, duration and time to determine the level of serum ALT. Results: ① The best way to set up the acute liver damage in rats was to give 10% CCl4 by intragastrical injection.② The best way to make the liver intoxication in mice was to give 20% CCl4 0.05 ml/10g by intragastrical injection once every three days.③The model of alcoholic liver disease in mice was induced by alcohol 0.1 ml/10g, once a day for 5 to 7 days. Conclusions: The concentration, dose and route of medication of CCl4 as well as the dose, times and duration of alcohol are studied in this experiment. But effective method has yet to be explored for making better models.