茵陈蒿提取物对高脂诱导大鼠增强胰岛素敏感性及抗脂肪肝作用的研究

目的:研究茵陈蒿提取物对高脂诱导大鼠增强胰岛素敏感性及抗脂肪肝的作用。方法:采用高脂、高糖饲料喂养大鼠,饲养8wk后测定大鼠空腹血糖(FBG)和2h后血糖(2hBG)值,并按血糖值将大鼠随机分为模型组、二甲双胍组(0.2 g·kg~(-1))及茵陈蒿提取物高(6.9g·kg~(-1))、低(2.3g·kg~(-1))剂量组。连续灌胃给药4wk后,测定大鼠FBG和血清胰岛素水平;测定血浆三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、游离脂肪酸(FFA)水平;测定天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性和抗氧化能力等;评估肝组织脂肪变性情况。结果:茵陈蒿提取物高剂量组大鼠FBG和血清胰岛素水平显著低于模型组,胰岛素敏感指数(ISI)和胰岛素抵抗指数(IRI)恢复正常(P<0.05);能显著提高超氧化物歧化酶(SOD)活性和降低丙二醛(MDA)含量,降低TC、TG、FFA和LDL-C水平,升高HDL-C水平;同时显著降低AST和ALT活性,改善肝脏脂肪病变。结论:茵陈蒿提取物对胰岛素抵抗(IR)-脂肪肝具有降低血脂和保肝作用,其作用机制可能与其提高抗氧化能力、恢复胰岛素敏感性有关。

Study on Artemisia Capillaris Thunb. Extract in Enhancing Insulin Sensitivity and Antagonizing Nonalcoholic Fatty Liver in High Fat-Fed Rats

OBJECTIVE:To study effects of Artemisia capillaris Thunb.extract on enhancing insulin sensitivity and an- tagonizing nonalcoholic fatty liver in high-fat fed rats.METHODS:Rats were fed with high-fat and high-sugar diet for 8 wk, then the fasting blood glucose(FBG)level and 2hBG in rats were determined.Based on the BG levels,the rats were randomly divided into model group,Metformin group(at a dose of 0.2 g·kg~(-1)),high dose Artemisia capillaris Thunb.extract group (6.9g·kg~(-1))and low dose Artemisia capillaris Thunb.extract group(2.3g·kg~(-1)).After intragastric administration with drugs for 4 consecutive weeks,levels of FBG,serum insulin,serum lipid(TC,TG,LDL-C,HDL-C,and FFA),and activities of AST,ALT and SOD etc were measured,and the fatty degeneration of liver tissues was evaluated.RESULTS:In rats treated with high dose Artemisia capillaris Thunb.extract compared with model rats,FBG and insulin levels were significantly lower,insulin sensitivity index(ISI)and insulin resistance index(IRI)were recovered to normal(P<0.05),activity of SOD was significantly increased,MDA content was significantly decreased,levels of serum lipid(TC,TG,FFA,and LDL-C)were lowered,level of HDL-C was increased,activities of AST and ALT were decreased significantly,and the pathological change of fatty liver was improved.CONCLUSION:Artemisia capillaris Thunb.extract can lower blood lipid and protect liver function in rats with insulin resistance(IR)-fatty liver,and this action mechanism may be attributed to the enhancing of antioxidation and recovery of insulin sensitivity.