肝细胞肝癌和肝硬化组织中丙型肝炎病毒各区段抗 …

目的 研究丙型肝炎病毒（ＨＣＶ）在肝细胞肝癌（ＨＣＣ）和肝硬化（ＬＣ）中所起的作用。结合乙型肝炎病毒（ＨＢＶ）进行分析，并初步探讨了ＨＣＶ与ＨＢＶ感染是否有相互促进作用。方法 采用ＨＣＶ－Ｃ，Ｅ，ＮＳ３，ＮＳ４区单克隆抗体，ＨＢｓＡｇ多克隆抗体用免疫组化方法检测了５９例ＨＣＣ及３５例ＬＣ组织标本。结果 ＨＣＶ阳性反应主要分布在肝细胞及癌细胞的胞浆内，呈细颗粒状。ＨＣＣ中，ＨＣＶ感染率；北京（２９例

Detection of hepatitis C virus-C, E, NS3 and NS4 regions' antigens in hepatocellular carcinoma and liver cirrhosis

Using 4 McAbs to HCV-C, E, NS3 and NS4 regions' antigens and PcAb to HBsAg, 59 cases of hepatocellular carcinoma(HCC) and 35 cases of liver cirrhosis(LC) were tested by immunohistochemistry technique. Positive reactions for hepatitis C virus were mainly present in the cytoplasm of hepatocytes and tumor cells with fine granules. The positive rates of HCV were 17 2% in HCC(29 cases) of Beijing, 26 7% in HCC(30 cases) of Shenyang and 14 3% in LC(35cases) of Shenyang. C region's McAb had the highest positive rate of detection, which suggested that C region's protein had a high level expression. The positive rates of hepatitis B virus surface antigen were 63 0% in HCC(29 cases) of Beijing, 73 3% in HCC(30 cases) of Shenyang and 54 3% in LC(35 cases) of Shenyang, all of which were higher than their positive rate for HCV detected. In HCC and LC, HBV and HCV inclined to suppress the opposite side.