脉络膜黑色素瘤细胞抗原的免疫组织化学观察

用单克隆抗体HMB-45对12例脉络膜黑色素瘤进行了免疫组织化学观察,结果表明全部病例与HMB-45呈阳性反应,反应强度十～不等,阳性颗粒呈棕色,位于胞浆中,而细胞膜和细胞核不着色,脉络膜黑色素瘤的不同细胞类型与反应强度有一定的关系。大多数病例与HMB-45呈不均匀性反应。脉络膜色素层的色素上皮全部与HMB-45呈阴性反应。结果表明HMB-45是一种特异性高,能与黑色素颗粒区别的可靠的标记物。

Immunohistochemical Observation of Choroidal Melanoma

12 cases with choroidal melanoma were studied by avidin-biotin complex (ABC) immunohistochemistry using monoclonal antibody HMB-45. The results show that all of 12 cases are positive for HMB-45 staining. Reaction intensity ranged from + to and positive granules localized in cytoplasm. Cell membranes and nucleis were nonreactive. Heterogeneity with HMB-45 was seen in most of cases. HMB-45 was negative in choroidal melanocytes. The results suggest that HMB-45 is a specific marker for diagnosis of choroidal melanoma.