胃肠道间质瘤中Ｍａｓｐｉｎ、ｕＰＡ、ＶＥＧＦ和ＶＥＧＦ-Ｃ的表达及其意义

目的　探讨胃肠道间质瘤（ＧＩＳＴ）组织中Ｍａｓｐｉｎ、ｕＰＡ、ＶＥＧＦ和ＶＥＧＦ-Ｃ的表达及临床意义。方法　应用免疫组织化学方法和逆转录ＰＣＲ法检测ＧＩＳＴ组织中Ｍａｓｐｉｎ、ｕＰＡ、ＶＥＧＦ及ＶＥＧＦ-Ｃ的表达情况，并分析各指标表达与ＧＩＳＴ生物学行为之间的关系以及Ｍａｓｐｉｎ与ｕＰＡ、ＶＥＧＦ、ＶＥＧＦ-Ｃ表达的相关性。结果　４２例ＧＩＳＴ组织中，Ｍａｓｐｉｎ、ｕＰＡ、ＶＥＧＦ和ＶＥＧＦ-Ｃ蛋白的表达率分别为６１.９％（２６／４２）、６４.３％（２７／４２）、５９.５％（２５／４２）和５４.８％（２３／４２）。逆转录ＰＣＲ检测显示，Ｍａｓｐｉｎ、ｕＰＡ、ＶＥＧＦ和ＶＥＧＦ-ＣｍＲＮＡ在ＧＩＳＴ组织中均有表达，且与其相应蛋白表达的趋势基本一致。随着ＧＩＳＴ侵袭危险性增加，Ｍａｓｐｉｎ表达显著降低，ｕＰＡ蛋白及ＶＥＧＦ、ＶＥＧＦ-Ｃ表达均显著增加（Ｐ＜０.０５），而ｕＰＡｍＲＮＡ表达无显著性差异（Ｐ＞０.０５）。随着ＧＩＳＴ出现复发转移，Ｍａｓｐｉｎ表达显著降低，ＶＥＧＦ和ＶＥＧＦ-Ｃ表达显著增加（Ｐ＜０.０５），而ｕＰＡ表达无显著性差异（Ｐ＞０.０５）。Ｍａｓｐｉｎ与ｕＰＡ、ＶＥＧＦ、ＶＥＧＦ-Ｃ表达均呈显著负相关（ｒ＝－０.４２９，Ｐ＜０.０５；ｒ＝－０.３１２，Ｐ＜０.０５；ｒ＝－０.２９２，Ｐ＜０.０５）。结论　Ｍａｓｐｉｎ表达下调或缺失促进ｕＰＡ、ＶＥＧＦ和ＶＥＧＦ-Ｃ表达上调在ＧＩＳＴ演进过程中可能扮演重要角色，４种蛋白均有可能是预测ＧＩＳＴ生物学行为及预后的重要辅助因子；Ｍａｓｐｉｎ基因有可能是ＧＩＳＴ靶向治疗新的研究靶点。

Expression and clinicopathological significance of maspin,uPA,VEGF and VEGF-C in patients with gastrointestinal stromal tumor

Objective To investigate the expression and clinical significance of maspin,uPA,VEGF and VEGF-C in gastrointestinal stromal tumor(GIST) patients.Methods The expression of maspin,uPA,VEGF and VEGF-C were detected by immunohistochemistry and RT-PCR,respectively.The relations between the expression of each factor and biological behavior and the correlation among maspin and uPA,VEGF as well as VEGF-C were respectively analyzed.Results In 42 GISTs tissues,the protein expression rates of maspin,uPA,VEGF and VEGF-...