葛根素对人骨肿瘤细胞株 MG63 的增殖抑制和诱导凋亡作用

目的 探讨葛根素对人骨肉瘤细胞株MG63的作用及其机制。方法 取对数生长期的MG63细胞分别用不同浓度的葛根素处理(0，25，50，100 mmol·L^-1)。细胞培养48 h后，利用MTT检测MG63细胞增殖水平；流式细胞术检测细胞凋亡水平，RT-PCR检测PI3K、AKT、Bax、Bcl-2、p53 和 p21 mRNA水平。Western blotting 检测PI3K、AKT、Bax、 Bcl-2、p53和p21表达水平。结果 葛根素浓度依赖性的诱导MG63细胞增殖抑制、凋亡和 Bax 表达，减少PI3K、AKT、Bcl-2、p53和p21表达。结论 葛根素可通过抑制PI3K/AKT/p53信号通路而诱导MG63细胞增殖抑制和凋亡。

Effect of Puerarin on Proliferation Inhibition and Apoptosis of Human Osteosarcoma Cell Lines MG63

OBJECTIVE To investigate the effect and mechanisms of puerarin on the human osteosarcoma cell lines MG63. METHODS MTT method was used to evaluate the proliferation, and the percentage of apoptotic cells were determined by flow cytometric analysis. The mRNA level of PI3K, AKT, Bax, Bcl-2, p53 and p21 were detected by RT-PCR. The expression of PI3K, AKT, Bax, Bcl-2, p53 and p21 were exanimed by Western blotting. RESULTS The results showed that puerarin treatment could induce the proliferation inhibition and apoptosis of MG63 cell in a concentration-dependent manner with the up-regulating the expression of Bax and down-regulating the expression of PI3K, AKT, Bcl-2, p53 and p21 in MG63 cell. CONCLUSION Puerarin can induce apoptosis and growth inhibition of human osteosarcoma cell lines MG63 and the mechanism is associated with suppressing PI3K/AKT/p53 signal pathway.