凤丹内生菌的分离鉴定及抑菌活性研究

从药用植物凤丹根部分离、纯化内生菌,运用形态学与分子生物学方法进行种类鉴定,采用生长速率法和滤纸片法进行抑菌活性研究。研究分离得到内生菌129株,其中内生真菌4属、6种、58株,优势属为镰刀菌属Fusarium;内生细菌3属、9种、71株,芽孢杆菌属Bacillus为优势属。Pseudomonas chlororaphis,F.nematophilum,Bacillus megaterium内生菌发酵液各自对金色葡萄球菌、大肠杆菌以及枯草杆菌的抑菌圈直径分别达到25.0,20.2,24.0 mm,F.nematophilum对青霉、黑线炭疽菌的抑菌率分别到达了90.6%,83.3%,P.chlororaphis对西瓜枯萎病原菌以及毛霉的抑制效果好,抑菌率分别为80.0%,84.9%。凤丹内生菌组成丰富,P.chlororaphis和F.nematophilum是具有较好的杀菌剂出发菌种。

Isolation, identification and inhibition activity of endophytes in Fengdan

Endophytes were isolated and purified from the roots of medicinal plant Fengdan also known as Paeonia suffruticosa from Tongling region, Anhui province, China. Morphology and molecular biology methods were applied to indentify the endophyte strains. And methods of growth rate and filtering paper were also used for studying antibacterial/antifungal effects of the strains. As a result, 129 endophyte strains were isolated. Fifty-eight endophytic fungi strains were identified as 6 species in 4 genera and the dominant genus was Fusarium. Seventy-one endophytic bacteria strains were identified as 9 species in 3 genera and the dominant genus was Bacillus. Inhibitory diameter with endophytic fermenting liquid of Pseudomonas chlororaphis, F. nematophilum and B. megaterium from P. suffruticosa against Staphyloccocus aureus, Escherichia coli and B. subtilis reached 25.0,20.2,24.0 mm respectively. The inhibition rate of endophytic fermenting liquid from F. nematophilum against Penicillium sp. and Colletotrichum dematium reached 90.6% and 83.3%, respectively. The inhibition effect of P. chlororaphis against F. oxysporum f. sp. niveum and Mucor sp. was good and the antifungal rate reached 80.0% and 84.9%, respectively. P. suffruticosa in Tongling region contains abundant endophytes. P. chlororaphis and F. nematophilum are valuable species as starting strain about microbicide.