| 低温冷冻对人精子染色体的影响 |
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| 引用本文： | 蒲军,陈在贤. 低温冷冻对人精子染色体的影响[J]. 中华男科学杂志, 2002, 8(1): 45-47 |
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| 作者姓名： | 蒲军 陈在贤 |
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| 作者单位： | 重庆医科大学附属第一医院泌尿科,重庆,400016 |
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| 基金项目： | 四川省计划生育委员会基金资助 (970 0 13 ) |
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| 摘 要： | 目的 :评价低温冷冻对人精子染色体的影响。 方法 :用人精子与去透明带金黄地鼠卵异种体外授精技术制备精子染色体 ,通过比较冷冻前后及不同冷冻方法间精子染色体畸变率和性染色体比例来评价低温冷冻对人精子染色体的影响。 结果 :精子染色体畸变率在冷冻前为 9.4 0 % (11/ 117) ,经速冻法冷冻后为 7.4 8% (8/ 10 7) ,经缓冻法冷冻后为 8.74 % (9/ 10 3) ,3者差异无显著性 (P >0 .75 )。X精子与Y精子比例在 3者间也差异无显著性 (P >0 .90 )。 结论 :低温冷冻对人精子染色体没有产生影响
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| 关 键 词： | 精子 冷冻保存 染色体 |
| 文章编号： | 1009-3591(2002)01-0045-03 |
| 修稿时间： | 2001-03-26 |
The Effect of Cryopreservation on Human Sperm Chromoso me |
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| Jun PU,Zai Xian CHEN. The Effect of Cryopreservation on Human Sperm Chromoso me[J]. National journal of andrology, 2002, 8(1): 45-47 |
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| Authors: | Jun PU Zai Xian CHEN |
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| Affiliation: | Department of Urology, First Hospital, Chongqing Medical University, Chongqing 400016, China. |
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| Abstract: | Objectives: To evaluate the effects of cryopreservation on human sperm chromosome. Methods: Sperm chromosome were acquired using in vitro fertilization of zona free hamster oocytes and human sperm .The frequency of sperm chromosome anomalies and sex chromosomes ratio before and after freezing and with different freezing methods were compared. Results: There were no significant differences of frequency of sperm chromosome anomalies among fresh , fast frozen and slow frozen sperm (9.40%,7.48% and 8.74% ) ( P >0.75) or ratios of sex chromosomes ( P >0.90). Conclusions: These studies indicate that cryopreservation does not exert effects on human sperm chromosome. |
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| Keywords: | Sperm Cryopreservation Chromosome |
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