Caspase-3在出生后大鼠海马中的表达

目的 探讨Wistar大鼠海马生后发育过程中,活化的Caspase-3与凋亡之间的关系. 方法 应用免疫荧光方法观测活化的Caspase-3和赫斯特荧光染料33342(Hoechst 33342)在生后不同时期大鼠海马CA1、CA3区和齿状回(DG)中的表达情况. 结果 在CA1区,活化的Caspase-3的表达在生后7d(P7)达到高峰;在CA3区,P2达到高峰,然后逐渐减弱.在DG,P7后又有所增强,到P14达到高峰,并在所观测的其余时段维持此水平.观测的3个区的凋亡细胞数目都在P7达到高峰,然后逐渐减少. 结论 在大鼠海马生后发育过程中,活化的Caspase-3的表达存在特定的时空格局.活化的Caspase-3在CA1区与CA3区有丝分裂后期细胞和DG神经前体细胞中的作用和机制不同.

Expression of Caspase-3 in rat hippocampus during postnatal development

Objective To investigate the relationship between active Caspase-3 and apoptosis in Wistar rat hippocampus during postnatal development. Methods Immunofluorescent staining was applied to observe the expression of active Caspase-3 and Hoechst 33342 in the CA1, CA3 and dentate gyrus（DG）of rat hippocampus during postnatal development. Results The expression of active Caspase-3 reached a peak at postnatal day 7(P7) in CA1, at P2 in CA3, then decreased with age. Whereas in DG, active Caspase-3 expression increased slightly after P7, reaching a maximum at P14, and remained at high levels for the rest of the investigated period. In addition, the number of apoptotic cells in the three regions all reached maximum levels at P7, then decreased with age. Conclusion There are specific spatio temporal patterns of expression of active Caspase-3 in the postnatally developing rat hippocampal subregions and active Caspase-3 in the postmitotic neurons of CA1 and CA3 and in neuronal progenitor cells of DG may have distinct roles and mechanisms