Isolation and characterization of membrane proteins of Plasmodium berghei sporozoites |
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| Authors: | A N Vermeulen W F Roeffen J C Van Munster J H Meuwissen |
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| Affiliation: | Department of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24, 6500 HB Nijmegen, The Netherlands |
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| Abstract: | Two immunologically significant proteins, Sp53 and Sp110, have been isolated from the sporozoites of Plasmodium berghei ANKA strain using different extraction procedures. In gel filtration studies the physicochemical characteristics of Sp53 and Sp110 appeared to be somewhat different. Both polypeptides could be purified using Sephacryl S300 column chromatography. The possible relationship of both Sp53 and Sp110 with sporozoite proteins described by other investigators is discussed. |
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| Keywords: | Sporozoites Rodent malaria Surface labelling Membrane protein purification Surface sporozoite proteins |
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